Khetawat D, Ghosh T, Bhattacharya M K, Bhattacharya S K, Chakrabarti S
National Institute of Cholera and Enteric Diseases, Calcutta, India.
Virus Res. 2001 Apr;74(1-2):53-8. doi: 10.1016/s0168-1702(00)00246-x.
The gene coding for outer capsid protein, VP7 of rotaviruses detected among the children suffering from watery diarrhea in the eastern part of India was studied. The full length gene, coding for VP7 was synthesized from a local strain, WD33, by combined reverse transcriptase-polymerase chain reaction. A total of 42 fecal RNA out of 93 rotavirus-positive samples hybridized with this DNA, indicating the prevalence of this strain in the community. Comparison of the nucleotide and deduced amino acid sequence with the VP7 gene of other serotypes revealed that the local strain,WD33, resembled closely with G1 serotype, the homology being 94 and 97%, respectively. Multiplex PCR using the specific oligonucleotide primers also amplified the G1 specific DNA fragment. The overall change of 16 amino acids was noticed when compared with G1 specific prototype Wa strain of which nine amino acids are within the neutralization domain. However, the phylogenetic tree constructed by comparing the G1 strains from different countries demonstrated that this strain WD33 clustered as a distinct sub-lineage of lineage IV and not with Wa strain, that clustered with lineage III.
对在印度东部患水样腹泻的儿童中检测到的轮状病毒外衣壳蛋白VP7的编码基因进行了研究。通过逆转录聚合酶链反应从本地毒株WD33合成了编码VP7的全长基因。93份轮状病毒阳性样本中共有42份粪便RNA与该DNA杂交，表明该毒株在社区中流行。将该核苷酸和推导的氨基酸序列与其他血清型的VP7基因进行比较，发现本地毒株WD33与G1血清型非常相似，同源性分别为94%和97%。使用特异性寡核苷酸引物进行的多重PCR也扩增出了G1特异性DNA片段。与G1特异性原型Wa毒株相比，发现有16个氨基酸发生了总体变化，其中9个氨基酸位于中和域内。然而，通过比较来自不同国家的G1毒株构建的系统发育树表明，该毒株WD33聚为谱系IV的一个独特亚谱系，而不是与聚为谱系III的Wa毒株聚在一起。
