Chen Dong-mei, Qian Yuan, Zhang You, Chang Ru-xu
Capital Institute of Pediatrics, Beijing Municipal Laboratory of Infection and Immunity, Beijing 100020, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003 Jun;17(2):165-8.
To compare the difference of VP7 fragment at the nucleotide level between rotavirus Guangzhou field strain R97-196 and rotavirus Beijing field strain or prototype strains.
The VP7 fragment amplified from Guangzhou field strain R97-196 by RT-PCR was cloned into the T-A cloning vector pUCm-T and sequenced.
The VP7 fragment from Guangzhou field strain R97-196 was 1,062 bp in length and contained two open reading frames which is consistent with that reported in the literature. The sequence analysis revealed that the cDNA from R97-196 shared higher nucleotide and amino acid identities with Beijing field strain T73 (98% and %, respectively) than with serotype 2-4 (G types) rotavirus (from 74% to 77% and 73% to 81%, respectively). The divergence of amino acid sequences is mainly within the nine divergence regions. The phylogenetic analysis revealed that the VP7 fragment from R97-196 was far away from rotavirus serotype G1 strain Wa.
The VP7 gene fragment from rotavirus Guangzhou field strain R97-196 belongs to rotavirus serotype G1. And variation of rotavirus VP7 gene fragment seems to be a geographic matter.
比较轮状病毒广州野毒株R97 - 196与轮状病毒北京野毒株或原型株在核苷酸水平上VP7片段的差异。
通过RT - PCR从广州野毒株R97 - 196扩增出VP7片段，克隆至T - A克隆载体pUCm - T并测序。
广州野毒株R97 - 196的VP7片段长度为1062 bp，包含两个开放阅读框，与文献报道一致。序列分析显示，R97 - 196的cDNA与北京野毒株T73的核苷酸和氨基酸同源性更高（分别为98%和[此处原文缺失具体百分比]），高于2 - 4型（G型）轮状病毒（核苷酸同源性分别为74%至77%，氨基酸同源性分别为73%至81%）。氨基酸序列差异主要存在于9个差异区域。系统发育分析显示，R97 - 196的VP7片段与轮状病毒G1血清型Wa株距离较远。
轮状病毒广州野毒株R97 - 196的VP7基因片段属于轮状病毒G1血清型。轮状病毒VP7基因片段的变异似乎与地理位置有关。
