DNA和RNA的伏安痕量分析。

Voltammetric trace analysis of DNA and RNA.

作者信息

Reher S, Lepka Y, Schwedt G

机构信息

Technical University of Clausthal, Institute of Inorganic and Analytical Chemistry, Clausthal-Zellerfeld, Germany.

出版信息

Fresenius J Anal Chem. 2000 Dec;368(7):720-6. doi: 10.1007/s002160000560.

DOI:10.1007/s002160000560

PMID:11227554

Abstract

A voltammetric determination of DNA/RNA is described. The new aspect is the use of the extracellular endonuclease Serratia marcescens in the sample preparation. Using this enzyme it is possible to determine DNA/ RNA with a detection limit of 2-5 pg/mL. This satisfies the requirements of the WHO and the FDA.

摘要

描述了一种伏安法测定DNA/RNA的方法。新的方面是在样品制备中使用细胞外核酸内切酶粘质沙雷氏菌。使用这种酶可以测定DNA/RNA，检测限为2-5 pg/mL。这满足了世界卫生组织和美国食品药品监督管理局的要求。