从粘质沙雷氏菌中快速提取高纯度染色体DNA。
Rapid extraction of high purity chromosomal DNA from Serratia marcescens.
作者信息
Alonso R, Nicholson P S, Pitt T L
机构信息
Servicio de Microbiología, Hospital Ramon y Cajal, Madrid, Spain.
出版信息
Lett Appl Microbiol. 1993 Feb;16(2):77-9. doi: 10.1111/j.1472-765x.1993.tb00348.x.
Abstract
Rapid non-specific degradation of Serratia marcescens DNA extracted with guanidium thiocyanate, occurred within 10 min of incubation with restriction endonuclease enzymes. The described modified method based on chemical and enzymatic deproteinization produced preparations of Ser. marcescens DNA of high yield and quality which did not autodegrade when incubated with restriction endonucleases.
摘要
用硫氰酸胍提取的粘质沙雷氏菌DNA在与限制性内切酶孵育10分钟内就会发生快速的非特异性降解。所描述的基于化学和酶促脱蛋白的改良方法产生了高产率和高质量的粘质沙雷氏菌DNA制剂，当与限制性内切酶孵育时不会自动降解。
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