隐孢子虫病康复期犊牛肠道黏膜中出现类似1型免疫反应的证据。

Evidence for the emergence of a type-1-like immune response in intestinal mucosa of calves recovering from cryptosporidiosis.

作者信息

Wyatt C R, Brackett E J, Savidge J

机构信息

Department of Veterinary Microbiology and Pathology, Washington State University College of Veterinary Medicine, Pullman 99164, USA.

出版信息

J Parasitol. 2001 Feb;87(1):90-5. doi: 10.1645/0022-3395(2001)087[0090:EFTEOA]2.0.CO;2.

DOI:10.1645/0022-3395(2001)087[0090:EFTEOA]2.0.CO;2

PMID:11227908

Abstract

This study was undertaken to characterize the mucosal response to Cryptosporidium parvum in infected calves that had recovered from diarrhea. Flow cytometric surface phenotypes of lamina propria lymphocyte (LPL) suspensions from infected calves and age-matched controls revealed the presence of a significantly larger proportion of CD25+ LPL in infected calves than in controls. Freshly isolated LPL from infected calves expressed more iNOS and interferon (IFN)-gamma than did controls. Infected calves excreted IgG1 and IgG2 isotype antibodies to C. parvum p23 by the end of the experiment. Moreover, immunohistochemistry of ileal sections revealed the presence of IgG1+ and IgG2+ B lymphocytes in the villi and IgG1+ but not IgG2+ B lymphocytes in continuous Peyer's patch nodules. These data are consistent with the emergence of a type-1-like mucosal immune response in terminal ileal mucosa as calves recover from cryptosporidiosis.

摘要

本研究旨在描述从腹泻中恢复的感染小牛对微小隐孢子虫的黏膜反应特征。对感染小牛和年龄匹配对照的固有层淋巴细胞（LPL）悬液进行流式细胞术表面表型分析，结果显示，感染小牛中CD25⁺LPL的比例显著高于对照。与对照相比，从感染小牛新鲜分离的LPL表达更多的诱导型一氧化氮合酶（iNOS）和干扰素（IFN）-γ。到实验结束时，感染小牛排泄出针对微小隐孢子虫p23的IgG1和IgG2同种型抗体。此外，回肠切片的免疫组织化学显示，绒毛中有IgG1⁺和IgG2⁺B淋巴细胞，连续的派尔集合淋巴结小结中有IgG1⁺但无IgG2⁺B淋巴细胞。这些数据与小牛从隐孢子虫病恢复时末端回肠黏膜中出现1型样黏膜免疫反应一致。