Canals A, Pasquali P, Zarlenga D S, Fayer R, Almeria S, Gasbarre L C
Immunology and Disease Resistance Laboratory, LPSI, ARS, USDA, Beltsville, Maryland 20705, USA.
J Parasitol. 1998 Feb;84(1):125-30.
In the present study, localized changes in cytokine transcription profiles were examined in neonatal calves following a primary infection with Cryptosporidium parvum, using competitive reverse transcriptase polymerase chain reaction (RT-PCR). Total RNA was prepared from ileocecal lymph nodes (LN), lamina propria lymphocytes (LPL), and intraepithelial lymphocytes (IEL) isolated from neonatal calves 7 days after C. parvum infection. Competitive RT-PCR performed on cDNA samples containing internal cytokine gene competitor molecules showed increases in the levels of interferon-gamma and interleukin-12 (IL-12) (P40) mRNA in both LPL and IEL populations but not in the draining LN. In addition, the levels of mRNA of the newly identified growth factor IL-15 decreased in the IEL of the infected animals. No consistent differences were seen in any of the cell populations when the samples were analyzed for IL-10 and levels of mRNA for IL-2 and IL-4 were low and highly variable in both infected and control groups in all 3 lymphocyte populations.
在本研究中，利用竞争性逆转录聚合酶链反应（RT-PCR）检测了新生犊牛初次感染微小隐孢子虫后细胞因子转录谱的局部变化。在微小隐孢子虫感染7天后，从新生犊牛分离出回盲部淋巴结（LN）、固有层淋巴细胞（LPL）和上皮内淋巴细胞（IEL），并从中提取总RNA。对含有内部细胞因子基因竞争分子的cDNA样本进行竞争性RT-PCR分析，结果显示，LPL和IEL群体中γ干扰素和白细胞介素-12（IL-12）（P40）mRNA水平升高，但引流淋巴结中未升高。此外，新鉴定的生长因子IL-15的mRNA水平在感染动物的IEL中降低。当分析样本中的IL-10时，在所有3个淋巴细胞群体的感染组和对照组中，IL-2和IL-4的mRNA水平均较低且高度可变，在任何细胞群体中均未观察到一致的差异。
