Zhong X Y, Laivuori H, Livingston J C, Ylikorkala O, Sibai B M, Holzgreve W, Hahn S
Laboratory for Prenatal Medicine, Department of Obstetrics and Gynaecology, University of Basel, Switzerland.
Am J Obstet Gynecol. 2001 Feb;184(3):414-9. doi: 10.1067/mob.2001.109594.
Elevated amounts of circulating fetal deoxyribonucleic acid in maternal plasma have recently been detected in pregnancies complicated by preeclampsia. We attempted to confirm this finding and simultaneously examined the quantity of maternal circulating deoxyribonucleic acid.
Circulating deoxyribonucleic acid was measured by realtime quantitative polymerase chain reaction in plasma samples obtained from 44 women with preeclampsia and a matched cohort of 53 normotensive pregnant women.
We confirmed that circulating fetal deoxyribonucleic acid levels were significantly elevated in pregnancies complicated by preeclampsia (3194.6 vs 332.8 copies/mL; P < .001). We also showed for the first time that circulating maternal deoxyribonucleic acid levels are also elevated (219,023.9 vs 20,235.8 copies/mL; P < .001). The increases in these deoxyribonucleic acid levels corresponded to the severity of the disorder, and values were correlated with each other in pregnancies complicated by preeclampsia (r = 0.556; P < .001) but not normotensive pregnancies (r = 0.046; P = .747).
The releases of both free fetal and maternal deoxyribonucleic acid were found to be affected in preeclampsia.
最近在子痫前期合并妊娠中检测到母体血浆中循环胎儿脱氧核糖核酸含量升高。我们试图证实这一发现，并同时检测母体循环脱氧核糖核酸的量。
采用实时定量聚合酶链反应法检测44例子痫前期患者及53例血压正常的孕妇匹配队列血浆样本中的循环脱氧核糖核酸。
我们证实，子痫前期合并妊娠中循环胎儿脱氧核糖核酸水平显著升高（3194.6对332.8拷贝/毫升；P<.001）。我们还首次表明，循环母体脱氧核糖核酸水平也升高（219,023.9对20,235.8拷贝/毫升；P<.001）。这些脱氧核糖核酸水平的升高与疾病的严重程度相关，并且在子痫前期合并妊娠中两者的值相互关联（r = 0.556；P<.001），但在血压正常的妊娠中无相关性（r = 0.046；P = .747）。
发现子痫前期中游离胎儿和母体脱氧核糖核酸的释放均受到影响。
