母血循环中细胞外胎儿DNA浓度与子痫前期严重程度的相关性分析。
The analysis of the correlation between extracellular fetal DNA concentration in maternal circulation and severity of preeclampsia.
作者信息
Engel Karina, Płonka Tomasz, Bilar Marek, Czajkowska Ewa, Orzińska Ewa, Brojer Ewa, Ronin-Walknowska Elzbieta
机构信息
Klinika Medycyny Matczyno-Płodowej Pomorskiej Akademii Medycznej w Szczecinie, Szczecin.
出版信息
Ann Acad Med Stetin. 2007;53(3):20-5.
INTRODUCTION
Increased fetal DNA in maternal plasma has been reported in pregnancies complicated by preeclampsia. Fetal DNA may by liberated from fetal or placental cells during apoptosis. The aim of the study is the estimation of the correlation between clinical and biochemical characteristics of preeclampsia and the concentration of fetal DNA in maternal circulation.
MATERIAL AND METHODS
Peripherial blood samples were obtained from women suffering from preeclampsia and healthy pregnant women between 20 and 28 weeks of pregnancy. For analysis of DNA isolated from maternal plasma real time PCR were performed.
CONCLUSIONS
- Significant increase of fetal DNA concentration in preeclamptic women plasma is associated with severe course of preeclampsia and especially with fetal distress symptoms. 2. The evaluation of number of fetal DNA copies in pregnant women plasma is characterized by high sensitivity but low specificity of this test in regards to preeclampsia with complications such as HELLP syndrome and intrauterine growth retardation (IUGR).
引言
据报道，在患有先兆子痫的妊娠中，母血中胎儿DNA水平会升高。胎儿DNA可能在细胞凋亡过程中从胎儿或胎盘细胞中释放出来。本研究的目的是评估先兆子痫的临床和生化特征与母血循环中胎儿DNA浓度之间的相关性。
材料与方法
在妊娠20至28周期间，从患有先兆子痫的妇女和健康孕妇中采集外周血样本。对从母血中分离出的DNA进行实时聚合酶链反应分析。
结论
- 先兆子痫妇女血浆中胎儿DNA浓度的显著升高与先兆子痫的严重病程相关，尤其是与胎儿窘迫症状相关。2. 孕妇血浆中胎儿DNA拷贝数的评估具有高敏感性，但对于伴有HELLP综合征和宫内生长受限（IUGR）等并发症的先兆子痫，该检测的特异性较低。
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