Grunditz C, Dalhammar G
Department of Biotechnology, Royal Institute of Technology (KTH), SE-100 44 Stockholm, Sweden.
Water Res. 2001 Feb;35(2):433-40. doi: 10.1016/s0043-1354(00)00312-2.
Restricted requirements for nitrogen reduction at wastewater treatment plants have increased the need for assays determining the inhibition of nitrification. In this paper, two new assays studying ammonia oxidation and nitrite oxidation, respectively, are presented. As test organisms, pure cultures of Nitrosomonas and Nitrobacter isolated from activated sludge are used. The assays are performed in test tubes where the bacteria are incubated with the compound or wastewater to be tested. The nitrification rate is measured during 4 h and compared with reference samples. The test organisms were characterised with respect to temperature, pH and cell activity. Optimum temperature was 35 degrees C for Nitrosomonas and 38 degrees C for Nitrobacter; optimum pH was 8.1 for Nitrosomonas and 7.9 for Nitrobacter. There was a linear relationship between the nitrification rate and the cell concentration in the studied interval. The cell activity decreased slightly with storage time. A significant level of inhibition was calculated to 11% for the Nitrosomonas assay, and to 9% for the Nitrobacter assay. The assays are applicable to determination of nitrification inhibition in samples of industrial waste waters or influents of treatment plants, or chemical substances likely to be found in wastewater.
污水处理厂对氮减排的严格要求增加了对硝化抑制测定方法的需求。本文介绍了两种分别研究氨氧化和亚硝酸盐氧化的新方法。作为测试生物，使用从活性污泥中分离出的亚硝化单胞菌和硝化杆菌的纯培养物。这些方法在试管中进行，将细菌与待测试的化合物或废水一起孵育。在4小时内测量硝化速率，并与参考样品进行比较。对测试生物的温度、pH值和细胞活性进行了表征。亚硝化单胞菌的最佳温度为35℃，硝化杆菌为38℃；亚硝化单胞菌的最佳pH值为8.1，硝化杆菌为7.9。在所研究的区间内，硝化速率与细胞浓度之间存在线性关系。细胞活性随储存时间略有下降。计算得出亚硝化单胞菌测定法的显著抑制水平为11%，硝化杆菌测定法为9%。这些方法适用于测定工业废水样品、处理厂进水或废水中可能存在的化学物质中的硝化抑制作用。
