Kanwal A, Paraszkiewicz K, Długoński J
Department of Industrial Microbiology, University of Lódź, Poland.
Microbios. 2001;104(407):27-38.
Genetic analysis of Curvularia lunata IM 2901 transformants, previously obtained by electroporation with plasmid pAN7-1, was carried out. Isolates displayed several differences in hygromycin B resistance and their physiology. It was shown that plasmid pAN7-1 was integrated in different copy numbers and at different positions in the genome of the strains studied. Both the wild type and pAN7-1 isolates, when growing in liquid media, produced an extracellular emulsifying agent. The transformants differed in their growth kinetics, intensity of surfactant production and in the efficiency of cortexolone 11beta-hydroxylation, in comparison with the wild type. The micro-organisms varied in susceptibility to the lytic enzyme complex (Novozyme 234), which indicated the presence of differences in their cell wall composition and/or in architecture caused by an integrated plasmid pAN7-1.
对之前通过用质粒pAN7 - 1进行电穿孔获得的新月弯孢霉IM 2901转化体进行了遗传分析。分离株在潮霉素B抗性及其生理特性方面表现出若干差异。结果表明，质粒pAN7 - 1以不同的拷贝数整合在所研究菌株基因组的不同位置。野生型和pAN7 - 1分离株在液体培养基中生长时均产生一种细胞外乳化剂。与野生型相比，转化体在其生长动力学、表面活性剂产生强度以及皮质酮11β - 羟化效率方面存在差异。这些微生物对裂解酶复合物（诺维信234）的敏感性各不相同，这表明整合的质粒pAN7 - 1导致它们的细胞壁组成和/或结构存在差异。
