稻瘟病菌Magnaporthe grisea对潮霉素B的抗性转化
Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance.
作者信息
Leung H, Lehtinen U, Karjalainen R, Skinner D, Tooley P, Leong S, Ellingboe A
机构信息
Department of Plant Pathology, University of Wisconsin, Madison 53706.
出版信息
Curr Genet. 1990 May;17(5):409-11. doi: 10.1007/BF00334519.
PMID:2357737
Abstract
Low frequency, integrative transformation of three fertile hermaphroditic strains of Magnaporthe grisea has been achieved using plasmid pAN7-1 and cosmid pAN7-2, which contain an Escherichia coli hygromycin B phosphotransferase gene linked to Aspergillus nidulans regulatory sequences.
摘要
利用质粒pAN7 - 1和粘粒pAN7 - 2，已实现对稻瘟病菌三个可育两性菌株的低频整合转化，这两种质粒含有与构巢曲霉调控序列相连的大肠杆菌潮霉素B磷酸转移酶基因。
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