Varel V H, Miller D N
Roman L. Hruska U.S. Meat Animal Research Center, Agricultural Research Service, USDA, Clay Center, Nebraska 68933, USA.
Appl Environ Microbiol. 2001 Mar;67(3):1366-70. doi: 10.1128/AEM.67.3.1366-1370.2001.
Carvacrol and thymol in combination at 6.7 mM each completely inhibited the production of short-chain volatile fatty acids and lactate from cattle waste in anoxic flasks over 23 days. Fecal coliforms were reduced from 4.6 x 10(6) to 2.0 x 10(3) cells per ml 2 days after treatment and were nondetectable within 4 days. Total anaerobic bacteria were reduced from 8.4 x 10(10) to 1.5 x 10(7) cells per ml after 2 days and continued to be suppressed to that level after 14 days. If the concentration of carvacrol or thymol were doubled (13.3 mM), either could be used to obtain the same inhibitory fermentation effect. We conclude that carvacrol or thymol may be useful as an antimicrobial chemical to control pathogens and odor in stored livestock waste.
香芹酚和百里香酚各以6.7 mM的浓度组合，在缺氧烧瓶中对牛粪中短链挥发性脂肪酸和乳酸的产生进行了23天的完全抑制。处理2天后，粪便大肠菌群从每毫升4.6×10⁶个细胞减少到2.0×10³个细胞，4天内检测不到。总厌氧菌在2天后从每毫升8.4×10¹⁰个细胞减少到1.5×10⁷个细胞，并在14天后继续被抑制到该水平。如果香芹酚或百里香酚的浓度加倍（13.3 mM），则二者均可用于获得相同的抑制发酵效果。我们得出结论，香芹酚或百里香酚可用作抗菌化学品，以控制储存的牲畜粪便中的病原体和气味。
