Breitschwerdt E B, Sontakke S, Cannedy A, Hancock S I, Bradley J M
Departments of Clinical Sciences and Farm Animal Health and Resource Management, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
J Clin Microbiol. 2001 Mar;39(3):879-82. doi: 10.1128/JCM.39.3.879-882.2001.
Very recently, Bartonella organisms have been isolated from large ruminants (deer, elk, and dairy and beef cattle) located in the United States and in France. In this study, we report the serologic, microbiologic, and molecular findings related to the isolation of a Bartonella species in North Carolina beef cattle and the detection of nanobacterial antigen using a commercially available enzyme-linked immunosorbent assay. Between August 1998 and September 1999, blood was collected from 38 cattle ranging in age from 1 month to 6.5 years. After a 1-month incubation period, a Bartonella sp. was isolated on a 5% rabbit blood agar plate from three of six EDTA blood samples. PCR amplification of the 16S rRNA gene from all three isolates resulted in a DNA sequence that was 100% identical to that of B. weissii 16S rRNA (GenBank no. AF199502). By IFA testing, 36 of 38 cattle had antibodies (> or =1:64) to Bartonella weissii (bovine origin) antigens. Nanobacterial antigen was detected in 22 of 22 serum samples. We conclude that infection with an organism similar or closely related to B. weissii can occur in North Carolina cattle and that although their actual existence is still controversial Nanobacterium antigens were detected with a commercially available test kit. The epidemiology, vector biology, and potential pathogenicity of these organisms in cattle deserve future consideration.
最近，在美国和法国的大型反刍动物（鹿、麋鹿以及奶牛和肉牛）中分离出了巴尔通体属微生物。在本研究中，我们报告了与在北卡罗来纳州肉牛中分离出一种巴尔通体菌种以及使用市售酶联免疫吸附测定法检测纳米细菌抗原相关的血清学、微生物学和分子学研究结果。在1998年8月至1999年9月期间，采集了38头年龄从1个月至6.5岁的牛的血液。经过1个月的培养期后，从6份乙二胺四乙酸（EDTA）血液样本中的3份样本里，在含有5%兔血的琼脂平板上分离出了一种巴尔通体菌种。对所有3株分离菌的16S rRNA基因进行PCR扩增，得到的DNA序列与魏氏巴尔通体16S rRNA（GenBank编号：AF199502）的序列100%相同。通过间接荧光抗体试验（IFA）检测，38头牛中有36头对魏氏巴尔通体（牛源）抗原产生了抗体（≥1:64）。在22份血清样本中的22份里检测到了纳米细菌抗原。我们得出结论，在北卡罗来纳州的牛中可能发生与魏氏巴尔通体相似或密切相关的微生物感染，并且尽管纳米细菌的实际存在仍存在争议，但使用市售检测试剂盒检测到了纳米细菌抗原。这些微生物在牛群中的流行病学、媒介生物学以及潜在致病性值得未来进一步研究。
