Cherry Natalie A, Maggi Ricardo G, Cannedy Allen L, Breitschwerdt Edward B
Intracellular Pathogens Research Laboratory, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, United States.
Vet Microbiol. 2009 Mar 30;135(3-4):308-12. doi: 10.1016/j.vetmic.2008.09.063. Epub 2008 Sep 21.
Although an organism primarily associated with non-clinical bacteremia in domestic cattle and wild ruminants, Bartonella bovis was recently defined as a cause of bovine endocarditis. The purpose of this study was to develop a B. bovis species-specific PCR assay that could be used to confirm the molecular prevalence of Bartonella spp. infection. Blood samples from 142 cattle were tested by conventional PCR targeting the Bartonella 16S-23S intergenic spacer (ITS) region. Overall, Bartonella DNA was detected in 82.4% (117/142) of the cattle using either Bartonella genus primers or B. bovis species-specific primers. Based upon size, 115 of the 117 Bartonella genus ITS PCR amplicons were consistent with B. bovis infection, which was confirmed by PCR using B. bovis species-specific primers and by sequencing three randomly selected, appropriately sized Bartonella genus PCR amplicons. By DNA sequencing, Bartonella henselae was confirmed as the two remaining amplicons, showing sequence similarity to B. henselae URBHLIE 9 (AF312496) and B. henselae Houston 1 (NC_005956), respectively. Following pre-enrichment blood culture of 12 samples in Bartonella alpha Proteobacteria growth medium (BAPGM) B. henselae infection was found in another three cows. Four of the five cows infected with B. henselae were co-infected with B. bovis. To our knowledge this study describes the first detection of B. henselae in any large ruminant species in the world and supports the need for further investigation of prevalence and pathogenic potential of B. henselae and B. bovis in cattle.
虽然牛巴尔通体主要与家养牛和野生反刍动物的非临床菌血症有关，但最近它被定义为牛心内膜炎的一个病因。本研究的目的是开发一种牛巴尔通体物种特异性PCR检测方法，可用于确认巴尔通体属感染的分子流行情况。使用针对巴尔通体16S - 23S基因间隔区（ITS）的常规PCR对142头牛的血液样本进行检测。总体而言，使用巴尔通体属引物或牛巴尔通体物种特异性引物，在82.4%（117/142）的牛中检测到了巴尔通体DNA。基于大小，117个巴尔通体属ITS PCR扩增子中的115个与牛巴尔通体感染一致，这通过使用牛巴尔通体物种特异性引物的PCR以及对三个随机选择的、大小合适的巴尔通体属PCR扩增子进行测序得到了证实。通过DNA测序，确认另外两个扩增子为亨氏巴尔通体，分别显示出与亨氏巴尔通体URBHLIE 9（AF312496）和亨氏巴尔通体休斯顿1（NC_005956）的序列相似性。在巴尔通体α变形菌生长培养基（BAPGM）中对12个样本进行预富集血培养后，在另外三头奶牛中发现了亨氏巴尔通体感染。感染亨氏巴尔通体的五头奶牛中有四头同时感染了牛巴尔通体。据我们所知，本研究描述了世界上首次在任何大型反刍动物物种中检测到亨氏巴尔通体，并支持进一步调查亨氏巴尔通体和牛巴尔通体在牛中的流行情况和致病潜力的必要性。
