Coenye T, Falsen E, Hoste B, Ohlén M, Goris J, Govan J R, Gillis M, Vandamme P
Int J Syst Evol Microbiol. 2000 Mar;50 Pt 2:887-899. doi: 10.1099/00207713-50-2-887.
A polyphasic taxonomic study was performed on a group of isolates tentatively identified as Burkholderia cepacia, Ralstonia pickettii or Ralstonia paucula (formerly known as CDC group IVc-2). The isolates were mainly cultured from sputum of cystic fibrosis patients or from soil. SDS-PAGE of whole-cell proteins and AFLP fingerprinting distinguished at least five different species, and this was confirmed by DNA-DNA hybridizations. 16S rDNA sequence analysis of representative strains indicated that these organisms belong to the beta-subclass of the Proteobacteria, with the genera Burkholderia and Ralstonia as closest neighbours. Based on genotypic and phenotypic characteristics, the organisms were classified in a novel genus, Pandoraea. The DNA base composition of the members of the new genus is between 61.2 and 64.3 mol%. This novel genus includes four new species, Pandoraea apista (the type species) (type strain is LMG 16407T), Pandoraea pulmonicola (type strain is LMG 18106T), Pandoraea pnomenusa (type strain is LMG 18087T) and Pandoraea sputorum (type strain is LMG 18819T), and Pandoraea norimbergensis (Wittke et al. 1997) comb. nov. (type strain is LMG 18379T). The available clinical data indicate that at least some of these organisms may cause chronic infection in, and can be transmitted amongst, cystic fibrosis patients.
对一组初步鉴定为洋葱伯克霍尔德菌、皮氏罗尔斯顿菌或 paucula 罗尔斯顿菌（以前称为 CDC Ⅳc-2 群）的分离株进行了多相分类研究。这些分离株主要从囊性纤维化患者的痰液或土壤中培养获得。全细胞蛋白的 SDS-PAGE 和 AFLP 指纹图谱鉴定出至少五个不同的物种，DNA-DNA 杂交证实了这一点。代表性菌株的 16S rDNA 序列分析表明，这些微生物属于变形菌门的β亚类，伯克霍尔德菌属和罗尔斯顿菌属是其最亲近的邻居。根据基因型和表型特征，这些微生物被分类为一个新属，潘多拉菌属。新属成员的 DNA 碱基组成在 61.2 至 64.3 mol%之间。这个新属包括四个新物种，阿皮斯塔潘多拉菌（模式种）（模式菌株为 LMG 16407T）、肺潘多拉菌（模式菌株为 LMG 18106T）、普诺梅努萨潘多拉菌（模式菌株为 LMG 18087T）和痰液潘多拉菌（模式菌株为 LMG 18819T），以及诺伊mbergensis 潘多拉菌（Wittke 等人，1997 年）组合新名（模式菌株为 LMG 18379T）。现有的临床数据表明，这些微生物中至少有一些可能在囊性纤维化患者中引起慢性感染并在患者之间传播。
