Pan Z, Selyanko A A, Hadley J K, Brown D A, Dixon J E, McKinnon D
Department of Neurobiology and Behavior and Department of Physiology and Biophysics, State University of New York at Stony Brook, Stony Brook, NY 11794, USA.
J Physiol. 2001 Mar 1;531(Pt 2):347-58. doi: 10.1111/j.1469-7793.2001.0347i.x.
The region of alternative splicing in the KCNQ2 potassium channel gene was determined by RNase protection analysis of KCNQ2 mRNA transcripts. Systematic analysis of KCNQ2 alternative splice variant expression in rat superior cervical ganglia revealed multiple variant isoforms. One class of KCNQ2 splice variants, those that contained exon 15a, was found to have significantly different kinetics to those of the other isoforms. These transcripts encoded channel subunits that, when co-expressed with the KCNQ3 subunit, activated and deactivated approximately 2.5 times more slowly than other isoforms. Deletion of exon 15a in these isoforms produced a reversion to the faster kinetics. Comparison of the kinetic properties of the cloned channel splice variants with those of the native M-current suggests that alternative splicing of the KCNQ2 gene may contribute to the variation in M-current kinetics seen in vivo.
通过对KCNQ2 mRNA转录本进行核糖核酸酶保护分析，确定了KCNQ2钾通道基因中的可变剪接区域。对大鼠颈上神经节中KCNQ2可变剪接变体表达的系统分析揭示了多种变体亚型。发现一类包含外显子15a的KCNQ2剪接变体，其动力学与其他亚型有显著差异。这些转录本编码的通道亚基，在与KCNQ3亚基共表达时，激活和失活的速度比其他亚型慢约2.5倍。在这些亚型中删除外显子15a会使动力学恢复到更快的状态。将克隆的通道剪接变体的动力学特性与天然M电流的动力学特性进行比较表明，KCNQ2基因的可变剪接可能导致体内M电流动力学的变化。
