Grüllich C, Duvoisin R M, Wiedmann M, van Leyen K
Molecular Pharmacology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
FEBS Lett. 2001 Jan 26;489(1):51-4. doi: 10.1016/s0014-5793(01)02080-4.
Mammalian cells are characterized by an endomembrane system. Nevertheless, some cells lose these membranes during their terminal differentiation, e.g. red blood cells and lens fiber cells of the eye. 15-Lipoxygenase is believed to be critical for this membrane degradation. Here we use cultivated rabbit reticulocytes in the presence or absence of a lipoxygenase inhibitor to provide further evidence for the importance of 15-lipoxygenase for the in vivo degradation of mitochondria. We find that inhibitor treatment retarded mitochondrial degradation, as shown by persistence of marker proteins and by direct visualization of mitochondria by electron microscopy.
哺乳动物细胞的特征是具有内膜系统。然而，一些细胞在终末分化过程中会失去这些膜，例如红细胞和眼睛的晶状体纤维细胞。15-脂氧合酶被认为对这种膜降解至关重要。在这里，我们使用在有或没有脂氧合酶抑制剂的情况下培养的兔网织红细胞，以进一步证明15-脂氧合酶对线粒体体内降解的重要性。我们发现抑制剂处理会延迟线粒体降解，这通过标记蛋白的持续存在以及通过电子显微镜直接观察线粒体得以证明。
