Watson A, Doherty F J
Department of Biochemistry, University Medical School, Queen's Medical Centre, Nottingham, U.K.
Biochem J. 1994 Mar 1;298 ( Pt 2)(Pt 2):377-83. doi: 10.1042/bj2980377.
The reticulocyte 15-lipoxygenase (linoleate:oxygen oxidoreductase, EC 1.13.11.12) is implicated in oxidative damage to reticulocyte mitochondria before their elimination by degradation during maturation to the erythrocyte. A proportion of the 15-lipoxygenase sediments with the mitochondrial-rich stromal fraction of density-gradient-fractionated rabbit reticulocytes suggesting a physical association with mitochondria before their elimination. Ca2+ promotes binding of reticulocyte 15-lipoxygenase to isolated rat liver and reticulocyte mitochondria and 15-lipoxygenase-mediated lipid peroxidation of mitochondrial lipids and free linoleic acid. Association of reticulocyte 15-lipoxygenase with isolated mitochondria is not simply a consequence of Ca(2+)-induced swelling, but implies that Ca2+ mediates translocation of soluble lipoxygenase to mitochondrial membranes. Therefore, Ca2+ may have an important physiological role in the regulation of 15-lipoxygenase-mediated targeting of reticulocyte mitochondria for degradation.
网织红细胞15 - 脂氧合酶（亚油酸：氧氧化还原酶，EC 1.13.11.12）在网织红细胞线粒体在成熟为红细胞过程中通过降解被清除之前，参与对其的氧化损伤。在密度梯度分离的兔网织红细胞富含线粒体的基质部分中，有一定比例的15 - 脂氧合酶沉降，这表明在其被清除之前与线粒体存在物理关联。Ca2 +促进网织红细胞15 - 脂氧合酶与分离的大鼠肝脏和网织红细胞线粒体结合，以及15 - 脂氧合酶介导的线粒体脂质和游离亚油酸的脂质过氧化。网织红细胞15 - 脂氧合酶与分离的线粒体的结合并非简单地是Ca(2 +)诱导肿胀的结果，而是意味着Ca2 +介导可溶性脂氧合酶向线粒体膜的转运。因此，Ca2 +可能在调节15 - 脂氧合酶介导的网织红细胞线粒体靶向降解中具有重要的生理作用。
