Hu Y K, Xin Z Y, Chen X, Zhang Z Y, Duan X Y
Institute of Crop Breeding and Cultivation, CAAS, Beijing 100081, China.
Yi Chuan Xue Bao. 2001;28(2):152-7.
Eleven seedlings with resistance and two amphidiploids with disease resistance at all developmental stage were screened by using No. 15 isolate of E. graminis f. sp. tritici to inoculate 99 T. durum-Ae. squarrosa amphidiploids. The two amphidiploids with disease resistance at all developmental stage, M53 and M81, and their Ae. squarrosa parents were resistant to No. 15 isolate of powdery mildew payhogen, but their T. durum parents were susceptible. Therefore, the powdery mildew resistance of M53 and M81 were derived from Ae. squarrosa. Amphidiploid M74, which had the same durum parent as M53 but different Ae. squrrosa parent, was susceptible to No. 15 isolate in adult stage. M29 and M35, which had the same durum parent as M81 but different Ae. squarrosa parent were susceptible in all stage. These results were also proved that the powdery mildew resistance genes were derived from Ae. sqarrosa. Genetic analysis showed that powdery mildew resistance gene in M53 or M81 was a single dominant gene. A series of lines with known powdery mildew resistance genes and two unknown lines were used to test response patterns with 14 differential isolates of E. graminis f. sp. tritici. The response patterns in M53 and M81 were different from Pm1 to Pm21 and indicated that M53 and M81 conferred a new powdery mildew resistance gene respectively.
用小麦白粉菌15号生理小种接种99份硬粒小麦-节节麦双二倍体，筛选出11份具有抗性的幼苗和2份在所有发育阶段均抗病的双二倍体。两个在所有发育阶段均抗病的双二倍体M53和M81及其节节麦亲本对白粉病病原菌15号生理小种具有抗性，但其硬粒小麦亲本易感病。因此，M53和M81的白粉病抗性源自节节麦。双二倍体M74与M53具有相同的硬粒小麦亲本，但节节麦亲本不同，在成株期对15号生理小种易感病。M29和M35与M81具有相同的硬粒小麦亲本，但节节麦亲本不同，在所有阶段均易感病。这些结果也证明了白粉病抗性基因源自节节麦。遗传分析表明，M53或M81中的白粉病抗性基因是单个显性基因。用一系列具有已知白粉病抗性基因的品系和两个未知品系对14个小麦白粉菌生理小种鉴别寄主进行反应型测定。M53和M81的反应型与Pm1至Pm21不同，表明M53和M81分别携带一个新的白粉病抗性基因。
