Phytopathology. 1998 Feb;88(2):144-7. doi: 10.1094/PHYTO.1998.88.2.144.
ABSTRACT A major gene for resistance to wheat powdery mildew (Blumeria graminis f. sp. tritici = Erysiphe graminis f. sp. tritici) has been successfully transferred into hexaploid common wheat (Triticum aestivum, 2n = 6x = 42, AABBDD) from wild einkorn wheat (Triticum monococcum subsp. aegilopoides, 2n = 2x = 14, AA). NC96BGTA5 is a germ plasm line with the pedigree Saluda x 3/PI427662. The response patterns for powdery mildew resistance in NC96BGTA5 were tested with 30 differential isolates of B. graminis f. sp. tritici, and the line was resistant to all tested isolates. The analyses of P(1), P(2), F(1), F(2), and BC(1)F(1) populations derived from NC96BGTA5 revealed two genes for wheat powdery mildew resistance in the NC96BGTA5 line. One gene, Pm3a, was from its recurrent parent Saluda, and the second was a new gene introgressed from wild einkorn wheat. The gene was determined to be different from Pm1 to Pm21 by gene-for-gene and pedigree analyses. The new gene was identified as linked to the Pm3a gene based on the F(2) and BC(1)F(1) populations derived from a cross between NC96BGTA5 and a susceptible cultivar NK-Coker 68-15, and the data indicated that the gene was located on chromosome 1A. It is proposed that this new gene be designated Pm25 for wheat powdery mildew resistance in NC96BGTA5. Three random amplified polymorphic DNA markers, OPX06(1050), OPAG04(950), and OPAI14(600), were found to be linked to this new gene.
已成功地将小麦白粉病（Blumeria graminis f. sp. tritici = Erysiphe graminis f. sp. tritici）的主效抗性基因从野生一粒小麦（Triticum monococcum subsp. aegilopoides，2n = 2x = 14，AA）导入六倍体普通小麦（Triticum aestivum，2n = 6x = 42，AABBDD）。NC96BGTA5 是一个种质系，其系谱为 Saluda x 3/PI427662。用 30 个不同的小麦白粉病 B. graminis f. sp. tritici 分离物测试 NC96BGTA5 的白粉病抗性反应模式，该系对所有测试的分离物均具有抗性。来自 NC96BGTA5 的 P(1)、P(2)、F(1)、F(2)和 BC(1)F(1)群体的分析表明，NC96BGTA5 系中存在两个小麦白粉病抗性基因。一个基因 Pm3a 来自其轮回亲本 Saluda，另一个则是从野生一粒小麦中导入的新基因。通过基因对基因和系谱分析，该基因被确定与 Pm1 到 Pm21 不同。根据 NC96BGTA5 与易感品种 NK-Coker 68-15 杂交产生的 F(2)和 BC(1)F(1)群体，将该新基因确定为与 Pm3a 基因连锁，数据表明该基因位于 1A 染色体上。建议将该新基因命名为 NC96BGTA5 中抗小麦白粉病的 Pm25。发现三个随机扩增多态性 DNA 标记 OPX06(1050)、OPAG04(950)和 OPAI14(600)与该新基因连锁。
