Kozak L P, Erdelsky K J
J Cell Physiol. 1975 Apr;85(2 Pt 2 Suppl 1):437-47. doi: 10.1002/jcp.1040850410.
In addition to reviewing the genetic regulation of L-glycerol 3-phosphate dehydrogenase during development in the mouse, new evidence is presented that the electrophoretic properties of L-glucerol 3-phosphate dehydrogenase in Mus castaneus are determined by an allele (d) at the Gdc-1 locus. Accordingly there are three alleles at the Gdc-1 locus; the b allele in C57BL/6J mice differs from the d allele in electrophoretic properties and the c allele in BALB/cJ mice differs from the d allele with respect to both heat denaturation and electrophoretic properties. Identical segregation patterns of the L-glycerol 3-phosphate dehydrogenase phenotypes in liver, kidney, and skeletal muscle from offspring of an F2 generation produced from parents with the c/d genotype suggest that the Gdc-1 locus is the major structural locus for L-glycerol 3-phosphate dehydrogenase in these tissues. Heart muscle was pooled from mice of the F2 generation with either c/c or d/d genotypes at the Gdc-1 locus as determined by analysis of liver L-glycerol 3-phosphate dehydrogenase. The chromatographic properties of L-glycerol 3-phosphate dehydrogenase from the heart muscle was determined on DEAE-cellulose ion exchange columns. The elusion profile of the L-glycerol 3-phosphate dehydrogenase indicates that the Gdc-1 locus is also the major structural locus in heart muscle.
除了回顾小鼠发育过程中L-3-磷酸甘油脱氢酶的遗传调控外，还提供了新的证据，即小家鼠中L-3-磷酸甘油脱氢酶的电泳特性由Gdc-1位点的一个等位基因（d）决定。因此，Gdc-1位点有三个等位基因；C57BL/6J小鼠中的b等位基因在电泳特性上与d等位基因不同，而BALB/cJ小鼠中的c等位基因在热变性和电泳特性方面均与d等位基因不同。由具有c/d基因型的亲本产生的F2代后代的肝脏、肾脏和骨骼肌中L-3-磷酸甘油脱氢酶表型的相同分离模式表明，Gdc-1位点是这些组织中L-3-磷酸甘油脱氢酶的主要结构位点。通过分析肝脏L-3-磷酸甘油脱氢酶确定，从Gdc-1位点具有c/c或d/d基因型的F2代小鼠中收集心肌。在DEAE-纤维素离子交换柱上测定了心肌中L-3-磷酸甘油脱氢酶的色谱特性。L-3-磷酸甘油脱氢酶的洗脱图谱表明，Gdc-1位点也是心肌中的主要结构位点。
