Namihira M, Honma S, Abe H, Masubuchi S, Ikeda M, Honmaca K
Department of Physiology, Hokkaido University School of Medicine, Sapporo, Japan.
Neuroreport. 2001 Mar 5;12(3):471-5. doi: 10.1097/00001756-200103050-00010.
Circadian expression, light-responsiveness and localization of clock genes, rPer1 and rPer2, were examined in the rat retina under constant darkness. A significant circadian variation was detected in rPer2 transcript levels with a peak at ZT14, but not in the rPer1. A light pulse given after constant darkness of 3 days increased both rPer1 and rPer2 expression phase-dependently, while rPer1 was induced at more times than rPer2. A major site of these gene expression within the retina was the inner nuclear layer. These findings indicate that rPer1 and rPer2 genes play different roles in the generation and regulation of circadian rhythms in the retina from those in the suprachiasmatic nucleus.
在持续黑暗条件下，研究了大鼠视网膜中生物钟基因rPer1和rPer2的昼夜节律表达、光反应性及定位。rPer2转录水平呈现显著的昼夜节律变化，在ZT14达到峰值，而rPer1则未出现这种变化。持续黑暗3天后给予的一个光脉冲，能使rPer1和rPer2的表达呈相位依赖性增加，其中rPer1的诱导时间比rPer2更多。视网膜内这些基因表达的主要部位是内核层。这些发现表明，rPer1和rPer2基因在视网膜昼夜节律的产生和调节中所起的作用，与在视交叉上核中的作用不同。
