Iwasaki H, Oku H, Toda T, Nasu T, Miyagi T, Chinen L
Laboratory of Applied Biochemistry, Faculty of Agriculture, University of The Ryukyus, Okinawa, Japan.
J Nutr Sci Vitaminol (Tokyo). 2000 Oct;46(5):235-9. doi: 10.3177/jnsv.46.235.
The present study describes the cholesterol absorption by hyperlipidemia atherosclerosis prone (LAP) Japanese quail to address their high susceptibility to experimental atherosclerosis. The apparent cholesterol absorption rate of LAP quail was compared with that of commercially available (CA) Japanese quail. After 14 d of cholesterol feeding by gavage, it was found that the cholesterol excretion of LAP quail was significantly lower than that of CA quail. The fecal excretion of bile acid and fat showed a similar tendency to that as shown with the case of cholesterol. The cholesterol feeding only increased the serum cholesterol level of LAP quail, and this trend holds true for the liver lipid concentration. The expression level of liver cholesterol 7alpha-hydroxylase mRNA showed no difference between LAP and CA strains under the conditions of cholesterol loading. These results showed that the cholesterol absorption by LAP quail is significantly higher than that by CA quail, which may reasonably explain the higher susceptibility of this strain to experimental atherosclerosis.
本研究描述了高脂血症动脉粥样硬化易感性（LAP）日本鹌鹑的胆固醇吸收情况，以探讨它们对实验性动脉粥样硬化的高易感性。将LAP鹌鹑的表观胆固醇吸收率与市售（CA）日本鹌鹑进行比较。经灌胃给予胆固醇14天后，发现LAP鹌鹑的胆固醇排泄量显著低于CA鹌鹑。胆汁酸和脂肪的粪便排泄情况与胆固醇的情况呈现相似趋势。仅给予胆固醇喂养只会增加LAP鹌鹑的血清胆固醇水平，肝脏脂质浓度也呈现相同趋势。在胆固醇负荷条件下，LAP和CA品系之间肝脏胆固醇7α-羟化酶mRNA的表达水平没有差异。这些结果表明，LAP鹌鹑的胆固醇吸收显著高于CA鹌鹑，这可以合理地解释该品系对实验性动脉粥样硬化的更高易感性。
