Inoue Y, Toda T, Igawa T, Tani T, Kimura Y
Fujii Memorial Research Institute, Otsuka Pharmaceutical Co. Ltd., Otsu, Japan.
Tohoku J Exp Med. 1995 Jan;175(1):1-13. doi: 10.1620/tjem.175.1.
A hyperlipidemia-and-atherosclerosis-prone (LAP) quail model was developed by dietary cholesterol feeding through genetic selection of commercially available (CA) Japanese quail. The response of serum lipids and the aortic wall to dietary cholesterol feeding was compared in CA and LAP quails. Ten groups were fed a combination diet with different levels of cholesterol and corn oil for 12 weeks. In CA quail, dietary feeding of cholesterol only failed to induce significant hypercholesterolemia or atherosclerotic lesion. The respective optimal dietary levels of cholesterol and corn oil to induce hyperlipidemia and atherosclerotic lesion, were 2% and 15%, respectively. Ad-libitum feeding of only 0.5% cholesterol without corn oil induced significant hypercholesterolemia and aortic atherosclerosis in LAP quail. The main proliferating cellular component of the aortic atherosclerotic lesion was phenotypically transformed fibroblasts from medial fibroblasts. These results suggest that the LAP quail is a useful animal model for the study of atherosclerosis.
通过对市售（CA）日本鹌鹑进行基因选择，采用高胆固醇饮食喂养建立了一种易患高脂血症和动脉粥样硬化（LAP）的鹌鹑模型。比较了CA鹌鹑和LAP鹌鹑血清脂质及主动脉壁对高胆固醇饮食喂养的反应。将十组鹌鹑喂以不同胆固醇水平和玉米油的组合饲料，持续12周。在CA鹌鹑中，仅通过饮食喂养胆固醇未能诱导出显著的高胆固醇血症或动脉粥样硬化病变。诱导高脂血症和动脉粥样硬化病变的胆固醇和玉米油的各自最佳饮食水平分别为2%和15%。仅自由采食0.5%胆固醇而不添加玉米油可在LAP鹌鹑中诱导出显著的高胆固醇血症和主动脉粥样硬化。主动脉粥样硬化病变的主要增殖细胞成分是来自中膜成纤维细胞的表型转化成纤维细胞。这些结果表明，LAP鹌鹑是研究动脉粥样硬化的有用动物模型。
