使用甲基百里酚蓝作为显色剂直接分光光度法测定药品中铋的流动注射分析流路

Flow injection manifold for the direct spectrophotometric determination of bismuth in pharmaceutical products using Methylthymol Blue as a chromogenic reagent.

作者信息

Themelis D G, Tzanavaras P D, Papadimitriou J K

机构信息

Department of Chemistry, Laboratory of Analytical Chemistry, Aristotle University of Thessaloniki, 54006 Thessaloniki, Greece.

出版信息

Analyst. 2001 Feb;126(2):247-50. doi: 10.1039/b008661o.

DOI:10.1039/b008661o

PMID:11235112

Abstract

A new simple and rapid flow injection method is reported for the direct determination of bismuth in pharmaceutical products. Methylthymol Blue (MTB) was used as a chromogenic reagent and the absorbance of the colored Bi(III)-MTB complex produced was monitored at 548 nm. The various chemical and physical variables were optimized and a study of interfering ions was also carried out. Linear calibration graphs were obtained from 0 to 100 mg l-1 Bi(m) (120 injections per hour). The precision was very good (sr = 1.3%) and the limit of detection was cL = 0.150 mg l-1. The average accuracy was also very good (er = 0.75%) and was evaluated by comparison of the results obtained with those claimed by the manufacturers. The method was found to be adequately selective, considering the ions that the samples contain.

摘要

报道了一种用于直接测定药品中铋的新型简单快速流动注射方法。采用甲基百里酚蓝（MTB）作为显色剂，在548 nm处监测所生成的Bi(III)-MTB络合物的吸光度。对各种化学和物理变量进行了优化，并对干扰离子进行了研究。从0至100 mg l-1 Bi(III)获得了线性校准曲线（每小时进样120次）。精密度非常好（sr = 1.3%），检测限为cL = 0.150 mg l-1。平均准确度也非常好（er = 0.75%），通过将所得结果与制造商声称的结果进行比较来评估。考虑到样品中所含的离子，该方法具有足够的选择性。