Xiao Y J, Schwartz B, Washington M, Kennedy A, Webster K, Belinson J, Xu Y
Department of Cancer Biology, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA.
Anal Biochem. 2001 Mar;290(2):302-13. doi: 10.1006/abio.2001.5000.
Lysophospholipids (lyso-PLs), including various glycerol-based and sphingosine-based lysophospholipids, play important roles in many biochemical, physiological, and pathological processes. The classical methods to analyze these lipids involve gas chromatography and/or high-performance liquid chromatography, which are time-consuming, cumbersome, and sometimes inaccurate due to the incomplete separation of closely related lipid species. We now describe the quantitative analysis of lyso-PLs in ascites samples from patients with ovarian cancer using electrospray ionization spectrometry. Three new classes of lyso-PL molecules are detected: alkyl-LPA, alkenyl-LPA, and methylated lysophosphatidylethanolamine. Importantly, the following lysophospholipid species are significantly increased in ascites from patients with ovarian cancer, compared to patients with nonmalignant diseases (e.g., liver failure): LPA (including acyl-, alkyl-, and alkenyl-LPA species), lysophosphatidylinositol, and sphingosylphosphorylcholine. Lysophosphorylcholine contents are also significantly different among ascitic fluids from the two groups of patients. However, the total phosphate content in ascites samples from patients with ovarian cancer is not significantly different compared to that from patients with nonmalignant disease.
溶血磷脂（lyso-PLs），包括各种基于甘油和基于鞘氨醇的溶血磷脂，在许多生物化学、生理和病理过程中发挥着重要作用。分析这些脂质的经典方法包括气相色谱法和/或高效液相色谱法，这些方法耗时、繁琐，而且由于密切相关的脂质种类分离不完全，有时不准确。我们现在描述使用电喷雾电离光谱法对卵巢癌患者腹水样本中的溶血磷脂进行定量分析。检测到三类新的溶血磷脂分子：烷基溶血磷脂酸（alkyl-LPA）、烯基溶血磷脂酸（alkenyl-LPA）和甲基化溶血磷脂酰乙醇胺。重要的是，与非恶性疾病（如肝衰竭）患者相比，卵巢癌患者腹水中以下溶血磷脂种类显著增加：溶血磷脂酸（包括酰基、烷基和烯基溶血磷脂酸种类）、溶血磷脂酰肌醇和鞘氨醇磷酸胆碱。两组患者腹水中溶血磷酸胆碱含量也有显著差异。然而，卵巢癌患者腹水样本中的总磷含量与非恶性疾病患者相比没有显著差异。
