Hassan-Walker A F, Vargas Cuero A L, Mattes F M, Klenerman P, Lechner F, Burroughs A K, Griffiths P D, Phillips R E, Emery V C
Department of Virology, Royal Free and University College Medical School, London, United Kingdom.
J Infect Dis. 2001 Mar 15;183(6):835-43. doi: 10.1086/319260. Epub 2001 Feb 15.
The effects of the immunocompromised state after liver transplantation on the frequency of cytomegalovirus-specific cytotoxic T lymphocytes (CTL) were investigated in 93 patients by using HLA class I tetrameric complexes corresponding to HLA-A0201, HLA-B0702, HLA-B0801, and HLA-B3501 refolded with peptides from the ppUL83 matrix protein. ppUL83 CTL frequencies were suppressed during the first 6 months after transplantation. Patients with >1 HLA-restricted response detected had high correlation among ppUL83 CD8(+) CTL frequencies restricted by different HLA haplotypes (Spearman's rho=.67; P<.0001). There was an inverse correlation among levels of the calcineurin inhibitor, tacrolimus, and ppUL83 CD8(+) CTL frequencies (r=-.31; P=.005), which is consistent with the presence of a large proportion (70%) of activated (CD38(+)) ppUL83 CD8(+) CTL within the population of HLA class I tetramer-positive cells.
通过使用与HLA-A0201、HLA-B0702、HLA-B0801和HLA-B3501重折叠的来自ppUL83基质蛋白肽段的HLA I类四聚体复合物，对93例患者肝移植后免疫功能低下状态对巨细胞病毒特异性细胞毒性T淋巴细胞（CTL）频率的影响进行了研究。移植后的前6个月，ppUL83 CTL频率受到抑制。检测到>1种HLA限制性反应的患者，不同HLA单倍型限制的ppUL83 CD8(+) CTL频率之间具有高度相关性（Spearman秩相关系数ρ = 0.67；P < 0.0001）。钙调神经磷酸酶抑制剂他克莫司的水平与ppUL83 CD8(+) CTL频率呈负相关（r = -0.31；P = 0.005），这与HLA I类四聚体阳性细胞群体中很大比例（70%）的活化（CD38(+)）ppUL83 CD8(+) CTL的存在一致。
