Nauck M, März W, Jarausch J, Cobbaert C, Sägers A, Bernard D, Delanghe J, Honauer G, Lehmann P, Oestrich E, von Eckardstein A, Walch S, Wieland H, Assmann G
University of Freiburg, Germany.
Clin Chem. 1997 Sep;43(9):1622-9.
We evaluated a new homogeneous assay for the measurement of HDL-cholesterol (HDL-C) in six European laboratories. The assay includes two reagents and is applicable to most autoanalyzers, which allows full automation. The total CVs of the new method ranged between 1.3% and 6.7%. Thereby determined HDL-C values were in good agreement with those obtained by precipitation with phosphotungstic acid/MgCl2 or by a combination of ultracentrifugation and precipitation (0.956 < r < 0.994). The assay was linear up to at least 1500 mg/L HDL-C. Hemoglobin did not interfere, whereas icteric samples with bilirubin > 100 mg/L showed discrepancies between the homogeneous and the precipitation assay. Lipemia up to total triglyceride concentrations of 8000 mg/L did not interfere with the homogeneous HDL-C assay. The homogeneous HDL-C assay was easy to handle and produced similar results in all laboratories participating in this study. This method will significantly facilitate the screening of individuals at increased risk for cardiovascular disease.
我们在六个欧洲实验室评估了一种用于测量高密度脂蛋白胆固醇（HDL-C）的新型均相测定法。该测定法包括两种试剂，适用于大多数自动分析仪，可实现完全自动化。新方法的总变异系数在1.3%至6.7%之间。由此测定的HDL-C值与通过磷钨酸/氯化镁沉淀法或超速离心与沉淀相结合的方法获得的值高度一致（0.956 < r < 0.994）。该测定法在HDL-C至少为1500 mg/L时呈线性。血红蛋白无干扰，而胆红素>100 mg/L的黄疸样本在均相测定法和沉淀测定法之间存在差异。总甘油三酯浓度高达8000 mg/L的脂血对均相HDL-C测定法无干扰。均相HDL-C测定法易于操作，在参与本研究的所有实验室中产生了相似的结果。这种方法将显著促进对心血管疾病风险增加个体的筛查。
