Chang J, Sigal L J, Lerro A, Taylor J
Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111-2497, USA.
J Virol. 2001 Apr;75(7):3469-73. doi: 10.1128/JVI.75.7.3469-3473.2001.
As early as 5 days after DNA copies of the hepatitis delta virus (HDV) genome or even in vitro-transcribed HDV RNA sequences were injected into the mouse tail vein using the hydrodynamics-based transfection procedure of F. Liu et al. (Gene Ther. 6:1258-1266, 1999), it was possible to detect in the liver by Northern analyses of RNA, immunoblots of protein, and immunostaining of liver sections what were considered typical features of HDV genome replication. This transfection strategy should have valuable applications for in vivo studies of HDV replication and pathogenesis and may also be useful for studies of other hepatotropic viruses.
早在采用F. 刘等人（《基因治疗》6:1258 - 1266，1999年）基于流体动力学的转染方法将丁型肝炎病毒（HDV）基因组的DNA拷贝或甚至体外转录的HDV RNA序列注入小鼠尾静脉后的5天，就能够通过RNA的Northern分析、蛋白质的免疫印迹以及肝组织切片的免疫染色在肝脏中检测到被认为是HDV基因组复制的典型特征。这种转染策略对于HDV复制和发病机制的体内研究应具有重要应用价值，并且可能对其他嗜肝病毒的研究也有用。
