McCarthy L, Szabo I, Nitsche J F, Pintar J E, Rogers T J
Department of Microbiology and Immunology, The Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, 3400 North Broad Street, Philadelphia, PA 19140, USA.
J Neuroimmunol. 2001 Mar 1;114(1-2):173-80. doi: 10.1016/s0165-5728(01)00248-x.
We have examined the chemotactic responsiveness of thymocytes to selective mu-, kappa-, and delta-opioid agonists. Our results show that developing T cells migrate in response to mu-, but not kappa- or delta-opioids. The mu-opioid response appears to be dependent on the classical mu-opioid receptor (MOR-1) since the chemotactic response is blocked by a selective mu-opioid antagonist, and is absent in thymocytes from MOR-1-deficient mice. Flow cytometric analysis of the mu-opioid responsive cells shows that these cells consist predominantly of highly immature CD4- CD8- T cells. These results represent the first demonstration of the functional expression of mu-opioid receptors by developing T cells.
我们研究了胸腺细胞对选择性μ、κ和δ阿片样物质激动剂的趋化反应性。我们的结果表明，正在发育的T细胞会对μ阿片样物质产生迁移反应，但对κ或δ阿片样物质无反应。μ阿片样物质反应似乎依赖于经典的μ阿片样物质受体（MOR-1），因为趋化反应被选择性μ阿片样物质拮抗剂阻断，且在MOR-1缺陷小鼠的胸腺细胞中不存在该反应。对μ阿片样物质反应性细胞的流式细胞术分析表明，这些细胞主要由高度未成熟的CD4-CD8-T细胞组成。这些结果首次证明了正在发育的T细胞功能性表达μ阿片样物质受体。
