Alkemper J, Voorhees P W
Department for Materials Science and Engineering, Northwestern University, 2225 North Campus Drive, Evanston, Illinois 60208-3108, U.S.A.
J Microsc. 2001 Mar;201(Pt 3):388-94. doi: 10.1046/j.1365-2818.2001.00832.x.
A method for serial sectioning is presented that allows one to take about 20 sections per hour with spacings in the range 1-20 microm between sections. The alignment of the cross-sections is done with a linear variable differential transformer; it is thus independent of the microstructure of the sample and does not rely upon markers implanted in the sample. The alignment errors as well as tilts and rotation errors between sections associated with the new method are found to be negligible. Once all the sections are captured in a computer a three-dimensional image can be constructed. This image can be viewed interactively and rotated, thus allowing the direct observation of three-dimensional shapes. It can further be used to determine a vast array of microstructural parameters including those that cannot be determined from planar sections. The technique is illustrated through the reconstruction of the microstructure of a cast standard aluminium alloy specimen.
本文介绍了一种连续切片方法，该方法能够以每小时约20个切片的速度进行切片，切片间距在1至20微米之间。横截面的对齐通过线性可变差动变压器完成；因此，它与样品的微观结构无关，也不依赖于植入样品中的标记。发现与新方法相关的切片之间的对齐误差以及倾斜和旋转误差可以忽略不计。一旦所有切片都在计算机中捕获，就可以构建三维图像。该图像可以交互式查看和旋转，从而可以直接观察三维形状。它还可以进一步用于确定大量微观结构参数，包括那些无法从平面切片中确定的参数。通过铸造标准铝合金试样的微观结构重建来说明该技术。
