Mejri N, Franscini N, Rutti B, Brossard M
Institute of Zoology, Neuchâtel, Switzerland.
Parasite Immunol. 2001 Feb;23(2):61-9. doi: 10.1046/j.1365-3024.2001.00356.x.
BALB/c mice were infested with Ixodes ricinus larvae, nymphs or adults. Expression of IL-4 and IFN-gamma mRNA in axillary and brachial draining lymph node cells were measured by competitive quantitative reverse transcription-polymerase chain reaction 9 days after the beginning of primary-infestation. IL-4 mRNA was always higher than that of IFN-gamma mRNA for all tick instars. Moreover, IL-4 mRNA expression progressively increased during nymphal primary-infestation with a high burst of expression 7 days after the beginning of infestation. No evolution of IFN-gamma mRNA expression was detected. Draining lymph node cells of infested BALB/c produced higher level of IL-4 than IFN-gamma following in vitro restimulation with adult tick saliva, salivary gland extract (SGE) or with five selected different chromatographic fractions of SGE. Anti-tick IgG1 antibodies but no IgG2a were detected in BALB/c pluri-infested with I. ricinus nymphs, which confirmed the Th2 polarization of the immune response.
用蓖麻硬蜱幼虫、若虫或成虫感染BALB/c小鼠。在初次感染开始9天后，通过竞争性定量逆转录-聚合酶链反应测定腋窝和臂部引流淋巴结细胞中IL-4和IFN-γ mRNA的表达。对于所有蜱虫龄期，IL-4 mRNA总是高于IFN-γ mRNA。此外，在若虫初次感染期间，IL-4 mRNA表达逐渐增加，在感染开始7天后出现高表达爆发。未检测到IFN-γ mRNA表达的变化。在用成年蜱唾液、唾液腺提取物（SGE）或SGE的五个选定不同色谱级分进行体外再刺激后，受感染的BALB/c小鼠的引流淋巴结细胞产生的IL-4水平高于IFN-γ。在用蓖麻硬蜱若虫多次感染的BALB/c小鼠中检测到抗蜱IgG1抗体，但未检测到IgG2a，这证实了免疫反应的Th2极化。
