Ganapamo F, Rutti B, Brossard M
Department of Immunology, University of Neuchátel, Switzerland.
Scand J Immunol. 1996 Oct;44(4):388-93. doi: 10.1046/j.1365-3083.1996.d01-327.x.
In BALB/c mice repeatedly infested with nymphal Ixodes ricinus ticks, lymphocytes from axillary and brachial lymph nodes which drain the tick attachment site produced significant levels of IL-2, TNF-alpha and GM-CSF when stimulated in vitro with Con A or anti-CD3 antibodies. Cytokine production by cells from lymph nodes of the opposite flank was equivalent to that of cells from uninfested mice. Nine days after the first infestation and IL-2, GM-CSF were produced primarily by the CD4+ T cells, while some other cell types contributed also to the TNF-alpha production. In mice repeatedly infested, a gradual increase of lymph node cell production of IL-2 was observed. The IL-2 levels regularly increased from the first to the third infestation compared to TNF-alpha levels which gradually decreased. The in vitro production of GM-CSF was not affected by successive infestations. Spleen lymphocytes from naive mice produced higher levels of IL-2 than lymphocytes from axillary and brachial lymph nodes. Both tick salivary gland extracts and D-mannose inhibited IL-2 production by these lymphocytes.
在被蓖麻硬蜱若虫反复叮咬的BALB/c小鼠中，引流蜱叮咬部位的腋窝和臂淋巴结中的淋巴细胞，在体外用刀豆蛋白A或抗CD3抗体刺激时，会产生显著水平的白细胞介素-2（IL-2）、肿瘤坏死因子-α（TNF-α）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）。来自对侧胁腹淋巴结的细胞产生的细胞因子与未受感染小鼠的细胞相当。首次感染九天后，IL-2和GM-CSF主要由CD4 + T细胞产生，而其他一些细胞类型也参与了TNF-α的产生。在反复感染的小鼠中，观察到淋巴结细胞产生IL-2逐渐增加。与TNF-α水平逐渐下降相比，从第一次感染到第三次感染，IL-2水平有规律地升高。连续感染对GM-CSF的体外产生没有影响。未接触过抗原的小鼠的脾脏淋巴细胞产生的IL-2水平高于腋窝和臂淋巴结的淋巴细胞。蜱唾液腺提取物和D-甘露糖均抑制这些淋巴细胞产生IL-2。
