Kakinuma T, Nakamura K, Wakugawa M, Mitsui H, Tada Y, Saeki H, Torii H, Asahina A, Onai N, Matsushima K, Tamaki K
Department of Dermatology, Faculty of Medicine, and the Department of Molecular Preventive Medicine, University of Tokyo, Tokyo, Japan.
J Allergy Clin Immunol. 2001 Mar;107(3):535-41. doi: 10.1067/mai.2001.113237.
Atopic dermatitis (AD) is a chronic and relapsing inflammatory skin disease characterized by the predominant infiltration of TH2-type cells in lesional skin. Thymus and activation-regulated chemokine (TARC/CCL17) is a chemokine that attracts CC chemokine receptor 4-positive (CCR4+) or CCR8+ cells.
The purpose of this study was to investigate the participation of TARC in AD.
We measured serum TARC levels in 40 patients with AD, 20 healthy control subjects, and 20 patients with psoriasis. We also examined disease activity by using SCORAD score; serum soluble E-selectin, soluble IL-2 receptor, IgE, and GM-CSF levels; and eosinophil numbers in peripheral blood, as well as correlations between TARC levels and these factors. The positivity of CCR4 of CD4+CD45RO+ cells in PBMCs was examined by using FACS analysis. Immunohistochemical staining of TARC and GM-CSF was performed in the lesional skin of patients with AD.
The serum TARC levels of patients with AD were significantly higher than those of healthy control subjects and patients with psoriasis. The serum TARC levels significantly correlated with eosinophil number (r = 0.61), SCORAD score (r = 0.60), and serum soluble E-selectin levels (r = 0.58) and weakly correlated with serum soluble IL-2 receptor levels (r = 0.34) in patients with AD. The TARC levels of patients with AD decreased after the treatment in accordance with the improvement of clinical symptoms. The CCR4 positivity of CD4+CD45RO+ cells in PBMCs of patients with AD was also higher than that of healthy control subjects. Immunohistochemical staining revealed that TARC was positive in keratinocytes in the epidermis and in vascular endothelial cells, T cells, and dendritic cells in the dermis.
Serum TARC levels are associated with disease activity of AD, and TARC may play an important role in the pathogenesis of AD.
特应性皮炎（AD）是一种慢性复发性炎症性皮肤病，其特征为病变皮肤中以TH2型细胞为主的浸润。胸腺和活化调节趋化因子（TARC/CCL17）是一种趋化因子，可吸引CC趋化因子受体4阳性（CCR4+）或CCR8+细胞。
本研究旨在探讨TARC在AD中的作用。
我们检测了40例AD患者、20例健康对照者和20例银屑病患者的血清TARC水平。我们还使用SCORAD评分评估疾病活动度；检测血清可溶性E选择素、可溶性白细胞介素-2受体、免疫球蛋白E和粒细胞-巨噬细胞集落刺激因子水平；以及外周血嗜酸性粒细胞数量，并分析TARC水平与这些因素之间的相关性。通过流式细胞术分析检测外周血单个核细胞（PBMC）中CD4+CD45RO+细胞CCR4的阳性率。对AD患者的病变皮肤进行TARC和粒细胞-巨噬细胞集落刺激因子的免疫组织化学染色。
AD患者的血清TARC水平显著高于健康对照者和银屑病患者。AD患者血清TARC水平与嗜酸性粒细胞数量（r = 0.61）、SCORAD评分（r = 0.60）和血清可溶性E选择素水平（r = 0.58）显著相关，与血清可溶性白细胞介素-2受体水平（r = 0.34）弱相关。AD患者治疗后随着临床症状的改善，TARC水平下降。AD患者PBMC中CD4+CD45RO+细胞的CCR4阳性率也高于健康对照者。免疫组织化学染色显示，TARC在表皮角质形成细胞以及真皮中的血管内皮细胞、T细胞和树突状细胞中呈阳性。
血清TARC水平与AD的疾病活动度相关，TARC可能在AD的发病机制中起重要作用。
