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使用D-Squame作为一种微创技术评估犬特应性皮炎中的皮肤免疫反应生物标志物。

Use of D-Squame as a Minimally Invasive Technique to Evaluate Skin Immune Response Biomarkers in Canine Atopic Dermatitis.

作者信息

Mosca Marion, Milhau Nadège, Legain Mélanie, Idée Adrien, Langon Xavier, Pin Didier

机构信息

Interactions Cells Environment, 2016. A104, VetAgro Sup, Dermatology Department, 69280 Marcy L'étoile, France.

Research and Development Derm Research, Royal Canin, 30470 Aimargues, France.

出版信息

Vet Sci. 2024 Dec 28;12(1):4. doi: 10.3390/vetsci12010004.

DOI:10.3390/vetsci12010004
PMID:39852879
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11769324/
Abstract

Evaluation of skin inflammation biomarkers in canine atopic dermatitis (AD) currently requires skin biopsies. Tape stripping has been shown to be a reliable technique to study biomarkers in the stratum corneum (SC) in humans. The aim of this study was to assess the immune response and identify biomarkers in the SC of dogs with canine AD using D-squame as a minimally invasive technique. Eight beagle dogs were epicutaneously sensitized to extract after tape stripping on sensitized site (S); twice a week for 49 days. Two sites were determined: lesional site (L) and non-lesional site (NL) on eight dogs affected spontaneously with AD. Adhesive tape strips D-Squame were applied on each site. Skin concentrations of 10 cytokines were analyzed with an ELISA kit. Our results revealed a significant increase of IL-13, IL-4, and TNF-α concentrations in S and L sites. Regarding IFN-γ, its concentration was significantly increased in L skin and increased but not significantly in S sites. All the alarmins were not differentially expressed except IL-33 in the S site. IL-31, IL-1β, and IL-10 were not detectable. D-squame seems to be a suitable technique to extract inflammatory cytokines from the SC of dogs, and IL-13, IL-4, TNF-α, and IFN-γ could be interesting biomarkers of canine AD.

摘要

目前，评估犬特应性皮炎（AD）中的皮肤炎症生物标志物需要进行皮肤活检。胶带剥离已被证明是一种研究人类角质层（SC）中生物标志物的可靠技术。本研究的目的是使用D-角质层取样器作为一种微创技术，评估患有犬AD的犬的角质层中的免疫反应并鉴定生物标志物。八只比格犬在致敏部位（S）进行胶带剥离后经皮致敏提取物；每周两次，共49天。确定了两个部位：八只自发患有AD的犬的皮损部位（L）和非皮损部位（NL）。在每个部位应用胶带条D-角质层取样器。用ELISA试剂盒分析10种细胞因子的皮肤浓度。我们的结果显示，S和L部位的IL-13、IL-4和TNF-α浓度显著增加。关于IFN-γ，其浓度在L皮肤中显著增加，在S部位增加但不显著。除S部位的IL-33外，所有警报素均无差异表达。未检测到IL-31、IL-1β和IL-10。D-角质层取样器似乎是一种从犬的角质层中提取炎性细胞因子的合适技术，并且IL-13、IL-4、TNF-α和IFN-γ可能是犬AD的有趣生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/79ac901f22da/vetsci-12-00004-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/48bcdfe89a72/vetsci-12-00004-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/e62ebafea4b1/vetsci-12-00004-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/e3130ee66ab2/vetsci-12-00004-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/9e741298e4c6/vetsci-12-00004-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/29e8724f1df6/vetsci-12-00004-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/72ad89a882ca/vetsci-12-00004-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/2b0a4f76a049/vetsci-12-00004-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/102dcf3446aa/vetsci-12-00004-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/79ac901f22da/vetsci-12-00004-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/48bcdfe89a72/vetsci-12-00004-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/e62ebafea4b1/vetsci-12-00004-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/e3130ee66ab2/vetsci-12-00004-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/9e741298e4c6/vetsci-12-00004-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/29e8724f1df6/vetsci-12-00004-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/72ad89a882ca/vetsci-12-00004-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/2b0a4f76a049/vetsci-12-00004-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/102dcf3446aa/vetsci-12-00004-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f43/11769324/79ac901f22da/vetsci-12-00004-g009.jpg

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本文引用的文献

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Immunoexpression of IL-33 in the different clinical aspects of canine atopic dermatitis.犬特应性皮炎不同临床方面的 IL-33 免疫表达。
Vet Immunol Immunopathol. 2024 Jul;273:110786. doi: 10.1016/j.vetimm.2024.110786. Epub 2024 May 26.
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PDE4D and miR-203 are promising biomarkers for canine atopic dermatitis.PDE4D 和 miR-203 是犬过敏性皮炎有前途的生物标志物。
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Macroscopic Skin Examination Can Determine the Number of Strips Necessary to Study the in Dogs.宏观皮肤检查可确定研究犬类所需的条带数量。
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