Rorive S, Belot N, Decaestecker C, Lefranc F, Gordower L, Micik S, Maurage C A, Kaltner H, Ruchoux M M, Danguy A, Gabius H J, Salmon I, Kiss R, Camby I
Department of Pathology, Erasmus University Hospital, Brussels, Belgium.
Glia. 2001 Mar 1;33(3):241-55. doi: 10.1002/1098-1136(200103)33:3<241::aid-glia1023>3.0.co;2-1.
Protein (lectin)-carbohydrate interaction is supposed to be relevant for tumor cell behavior. The aims of the present work are to investigate whether galectin-1 modulates migration/invasion features in human gliomas in vitro, whether it can be detected in human gliomas immunohistochemically, and whether its expression is attributable to certain glioma subgroups with respect to invasion and prognosis. For this purpose, we quantitatively determined (by computer-assisted microscopy) the immunohistochemical expression of galectin-1 in 220 gliomas, including 151 astrocytic, 38 oligodendroglial, and 31 ependymal tumors obtained from surgical resection. We also xenografted three human glioblastoma cell lines (the H4, U87, and U373 models) into the brains of nude mice in order to characterize the in vivo galectin-1 expression pattern in relation to tumor invasion of the normal brain parenchyma. In addition, we characterized the role in vitro of galectin-1 in U373 tumor astrocyte migration and kinetics. Our data reveal expression of galectin-1 in all human glioma types with no striking differences between astrocytic, oligodendroglial, and ependymal tumors. The level of galectin-1 expression correlated with the grade in the group of astrocytic tumors only. Furthermore, immunopositivity of high-grade astrocytic tumors from patients with short-term survival periods was stronger than that of tumors from patients with long-term survivals. In human glioblastoma xenografts, galectin-1 was preferentially expressed in the more invasive parts of these xenografts. In vitro experiments revealed that galectin-1 stimulates migration of U373 astrocytes.
蛋白质（凝集素）-碳水化合物相互作用被认为与肿瘤细胞行为相关。本研究的目的是调查半乳糖凝集素-1是否在体外调节人类胶质瘤的迁移/侵袭特性，是否能通过免疫组织化学方法在人类胶质瘤中检测到，以及其表达是否归因于某些与侵袭和预后相关的胶质瘤亚组。为此，我们通过计算机辅助显微镜定量测定了220例胶质瘤中半乳糖凝集素-1的免疫组织化学表达，这些胶质瘤包括151例星形细胞瘤、38例少突胶质细胞瘤和31例室管膜瘤，均取自手术切除标本。我们还将三个人类胶质母细胞瘤细胞系（H4、U87和U373模型）异种移植到裸鼠脑中，以表征与正常脑实质肿瘤侵袭相关的体内半乳糖凝集素-1表达模式。此外，我们还表征了半乳糖凝集素-1在U373肿瘤星形胶质细胞迁移和动力学中的体外作用。我们的数据显示，半乳糖凝集素-1在所有人类胶质瘤类型中均有表达，星形细胞瘤、少突胶质细胞瘤和室管膜瘤之间无明显差异。半乳糖凝集素-1的表达水平仅与星形细胞瘤组的分级相关。此外，短期生存期患者的高级别星形细胞瘤的免疫阳性率高于长期生存期患者的肿瘤。在人类胶质母细胞瘤异种移植中，半乳糖凝集素-1优先表达于这些异种移植更具侵袭性的部分。体外实验表明，半乳糖凝集素-1刺激U373星形胶质细胞的迁移。
