Marczewska J, Koziorowska J H, Anuszewska E L
Drug Institute, Warsaw, Poland.
Acta Pol Pharm. 2000 Nov-Dec;57(6):415-8.
We investigated survival of two kinds of human embryonic cells (CLV102, Lu106) and human melanoma cells (Mel8) exposed to exogenous iron and copper ions in the absence or in the presence of ascorbic acid, catalase and superoxide dismutase. Iron ions produced cytotoxicity towards both kinds of cells dependent on its concentration. Catalase suppressed the cytotoxicity induced by iron ions in Lu106 cells. whereas in CLV102 and Mel8 cells, was ineffective. By contrast, superoxide dismutase abolished the cytotoxicity of iron ions towards CLV102 cells, whereas in Lu106 and Mel8 cells, was ineffective. The mixture of iron ions with ascorbic acid was less cytotoxic than iron ions themselves or ascorbic acid itself, only in CLV102 and Lu106 cells. Ascorbic acid enhanced drastically cytotoxic effect of copper ions in all kinds of cells.
我们研究了两种人类胚胎细胞（CLV102、Lu106）和人类黑色素瘤细胞（Mel8）在不存在或存在抗坏血酸、过氧化氢酶和超氧化物歧化酶的情况下，暴露于外源铁离子和铜离子时的存活率。铁离子对这两种细胞产生细胞毒性，且取决于其浓度。过氧化氢酶抑制了铁离子在Lu106细胞中诱导的细胞毒性，而在CLV102和Mel8细胞中则无效。相比之下，超氧化物歧化酶消除了铁离子对CLV102细胞的细胞毒性，而在Lu106和Mel8细胞中则无效。仅在CLV102和Lu106细胞中，铁离子与抗坏血酸的混合物比铁离子本身或抗坏血酸本身的细胞毒性更小。抗坏血酸在所有类型的细胞中都极大地增强了铜离子的细胞毒性作用。
