Chervinsky D S, Lam D H, Zhao X F, Melman M P, Aplan P D
Department of Cancer Genetics, Roswell Park Cancer Institute, USA.
Leukemia. 2001 Jan;15(1):141-7. doi: 10.1038/sj.leu.2401997.
We have established a panel of nine immortal cell lines from T cell malignancies which arose in mice transgenic for the SCL and LMO1 genes. Cells from the primary malignancies initially grew very slowly in vitro, loosely attached to a stromal layer, before gaining the ability to proliferate independently. Upon gaining the ability to proliferate in the absence of a stromal layer, these cell lines grew rapidly, doubling every 14-23 h, to a very high density, approaching 10(7) cells/ml. Whereas the tumors which arise in SCL/LMO1 double transgenic mice are typically diploid or pseudodiploid, the cell lines were all grossly aneuploid, suggesting the possibility that additional genetic events were selected for in vitro. Given that SCL and LMO1 gene activation are both commonly seen in human patients with T cell acute lymphoblastic leukemia, these cell lines may be a useful in vitro model for the human disease.
我们已经从SCL和LMO1基因转基因小鼠中发生的T细胞恶性肿瘤建立了一个包含9个永生细胞系的细胞库。原发性恶性肿瘤的细胞最初在体外生长非常缓慢，松散地附着于基质层，之后才获得独立增殖的能力。在获得在无基质层情况下增殖的能力后，这些细胞系迅速生长，每14 - 23小时翻倍，达到非常高的密度，接近10(7)个细胞/毫升。虽然在SCL/LMO1双转基因小鼠中出现的肿瘤通常是二倍体或假二倍体，但这些细胞系均为明显的非整倍体，这表明在体外可能选择了其他遗传事件。鉴于SCL和LMO1基因激活在人类T细胞急性淋巴细胞白血病患者中都很常见，这些细胞系可能是人类疾病的一个有用的体外模型。
