Kagami Y, Jung J, Choi Y S, Osumi K, Nakamura S, Morishima Y, Seto M
Department of Hematology and Chemotherapy, Aichi Cancer Center Hospital, Nagoya, Japan.
Leukemia. 2001 Jan;15(1):148-56. doi: 10.1038/sj.leu.2402002.
A novel cell line, FLK-1, was established from bone marrow cells of a patient with follicular lymphoma by means of co-culture with follicular dendritic cell (FDC)-like cell line HK. Immunophenotypic analysis showed that FLK-1 expressed CD10, CD19, CD20, CD38, IgG and HLA-DR, which is a typical feature of germinal center B cells. Cytogenetic analysis of FLK-1 demonstrated t(14;18)(q32;q21) translocation involving BCL2 and immunoglobulin heavy chain genes. Especially noteworthy is that the growth of FLK-1 was found to be dependent on a FDC line, HK. When HK cells were removed from the culture, FLK-1 cells stopped growing and eventually died. An apoptotic mechanism appeared to be involved as indicated by the presence of chromosome condensation and DNA ladder formation. The culture experiment using micropore membranes showed that soluble factor(s) of HK cells supported the growth, while direct cell-to-cell contact appeared to be necessary for longterm cell proliferation. These findings suggest the importance of the micro-environment for follicular lymphoma cells to grow. The FLK-1 cell line may thus prove to be useful for studying the growth mechanism of follicular lymphoma and provide new insights into the pathogenesis of follicular lymphoma.
通过与滤泡树突状细胞（FDC）样细胞系HK共培养，从一名滤泡性淋巴瘤患者的骨髓细胞中建立了一种新的细胞系FLK-1。免疫表型分析显示，FLK-1表达CD10、CD19、CD20、CD38、IgG和HLA-DR，这是生发中心B细胞的典型特征。对FLK-1的细胞遗传学分析显示存在涉及BCL2和免疫球蛋白重链基因的t(14;18)(q32;q21)易位。特别值得注意的是，发现FLK-1的生长依赖于FDC系HK。当从培养物中去除HK细胞时，FLK-1细胞停止生长并最终死亡。如染色体浓缩和DNA梯带形成的出现所示，似乎涉及凋亡机制。使用微孔膜的培养实验表明，HK细胞的可溶性因子支持生长，而直接的细胞间接触似乎是长期细胞增殖所必需的。这些发现表明微环境对滤泡性淋巴瘤细胞生长的重要性。因此，FLK-1细胞系可能被证明对研究滤泡性淋巴瘤的生长机制有用，并为滤泡性淋巴瘤的发病机制提供新的见解。
