Department of Hematology, Toyota Kosei Hospital, Toyota, Aichi, Japan.
Department of Hematology and Cell Therapy, Aichi Cancer Center Hospital, Nagoya, Aichi, Japan.
J Clin Exp Hematop. 2021 Sep 10;61(3):145-151. doi: 10.3960/jslrt.20058. Epub 2021 Jul 31.
We established an IL-2 and IL-4 (IL2/4) - dependent adult T-cell leukemia/lymphoma (ATLL) cell line (YG-PLL) by adding poly-L-lysine (PLL) to the culture medium. YG-PLL originates from lymphoma cells and contains a defective HTLV-I proviral genome. Although YG-PLL cannot survive without IL-2/4, the follicular dendritic cell (FDC)-like cell line HK expressing OX40-ligand gene (OX40LHK) inhibited their death in the presence of soluble neutral polymers. After the prevention of cell death, YG-PLL proliferated on OX40LHK without IL2/4 in the presence of two kinds of positively or negatively charged polymers. In particular, dermatan sulfate and poly-L-histidine supported growth for more than 4 months. Therefore, the original lymphoma cells proliferated transiently in the presence of IL2/4, and their growth arrest was inhibited by the addition of PLL. Furthermore, YG-PLL lost IL2/4 dependency by the following 3-step procedure: preculture with IL2/4 and neutral polymers, 3-day culture with neutral polymer on OX40LHK to inhibit cell death, and co-culture with OX40LHK in the presence of the positively and negatively charged polymers. The extracellular environment made by soluble polymers plays a role in the growth of ATLL in vitro.
我们通过在培养基中添加多聚赖氨酸（PLL）建立了一个 IL-2 和 IL-4（IL2/4）依赖性成人 T 细胞白血病/淋巴瘤（ATLL）细胞系（YG-PLL）。YG-PLL 来源于淋巴瘤细胞，含有缺陷的 HTLV-I 前病毒基因组。虽然 YG-PLL 在没有 IL-2/4 的情况下无法存活，但表达 OX40 配体基因（OX40LHK）的滤泡树突状细胞（FDC）样细胞系 HK 可以在存在可溶性中性聚合物的情况下抑制其死亡。在阻止细胞死亡后，YG-PLL 在没有 IL2/4 的情况下在两种带正电荷或负电荷的聚合物存在下在 OX40LHK 上增殖。特别是硫酸皮肤素和聚 L-组氨酸支持超过 4 个月的生长。因此，原始淋巴瘤细胞在 IL2/4 的存在下短暂增殖，其生长被 PLL 的添加抑制。此外，YG-PLL 通过以下 3 步程序失去对 IL2/4 的依赖性：用 IL2/4 和中性聚合物预培养、在 OX40LHK 上用中性聚合物培养 3 天以抑制细胞死亡，以及在带正电荷和负电荷的聚合物存在下与 OX40LHK 共培养。可溶性聚合物形成的细胞外环境在体外 ATLL 的生长中起作用。
