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作为生物膜形成和耐药性指标的表达

Expression as an Indicator for Biofilm Formation and Drug Resistance.

作者信息

Deng Keke, Jiang Wei, Jiang Yanyu, Deng Qi, Cao Jinzhong, Yang Wenjie, Zhao Xuequn

机构信息

Department of Respiratory, Tianjin Third Central Hospital Branch, Tianjin, China.

Department of Infectious Disease, Tianjin First Central Hospital, School of Medicine, Nankai University, Tianjin, China.

出版信息

Front Microbiol. 2021 Apr 29;12:655242. doi: 10.3389/fmicb.2021.655242. eCollection 2021.

DOI:10.3389/fmicb.2021.655242
PMID:33995316
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8117015/
Abstract

Resistance caused by the formation of the () biofilm is one of the main reasons for antifungal therapy failure. Thus, it is important to find indicators that predict biofilm formation to provide evidence for the early prevention and treatment of the biofilms. In this study, samples were selected from septicemia that were sensitive to common antifungal agents. It was found that the agglutinin-like sequence 3 ( gene was differentially expressed in free, antifungal, drug-sensitive . The average gene expression was higher in the strains with biofilm formation than that in the strains without biofilm formation. Then, it was further confirmed that the rate of biofilm formation was higher in the high gene expression group than that in the low gene expression group. It was found that with biofilm formation was more resistant to fluconazole, voriconazole, and itraconazole. However, it maintained its sensitivity to caspofungin and micafungin and in mice. Further experiments regarding the prevention of biofilm formation were performed in mice, in which only caspofungin and micafungin prevented biofilm formation. These results suggest that the expression level of in may be used as an indicator to determine whether will form biofilms. The results also show that the biofilm formation of remained sensitive to caspofungin and micafungin, which may help to guide the selection of clinical antifungal agents for prevention and therapy.

摘要

由（）生物膜形成引起的耐药性是抗真菌治疗失败的主要原因之一。因此，寻找预测生物膜形成的指标对于为生物膜的早期预防和治疗提供依据很重要。在本研究中，从对常见抗真菌药物敏感的败血症中选取样本。发现凝集素样序列3（基因在游离、抗真菌、药物敏感的（）中差异表达。有生物膜形成的（）菌株中该基因的平均表达高于无生物膜形成的（）菌株。然后，进一步证实高（）基因表达组的生物膜形成率高于低（）基因表达组。发现有生物膜形成的（）对氟康唑、伏立康唑和伊曲康唑更耐药。然而，它在体外和小鼠体内对卡泊芬净和米卡芬净仍保持敏感。在小鼠中进行了关于预防（）生物膜形成的进一步实验，其中只有卡泊芬净和米卡芬净能预防（）生物膜形成。这些结果表明（）中（）的表达水平可作为判断（）是否会形成生物膜的指标。结果还表明（）的生物膜形成对卡泊芬净和米卡芬净仍敏感，这可能有助于指导临床抗真菌药物预防和治疗的选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8117015/cb1d11c21c1c/fmicb-12-655242-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8117015/f954c7e99525/fmicb-12-655242-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8117015/94c5d36379a3/fmicb-12-655242-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8117015/2251c97b5876/fmicb-12-655242-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8117015/e6e6bde4e4db/fmicb-12-655242-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8117015/cb1d11c21c1c/fmicb-12-655242-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8117015/f954c7e99525/fmicb-12-655242-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8117015/94c5d36379a3/fmicb-12-655242-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8117015/2251c97b5876/fmicb-12-655242-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8117015/e6e6bde4e4db/fmicb-12-655242-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e6f/8117015/cb1d11c21c1c/fmicb-12-655242-g005.jpg

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本文引用的文献

[1]
Zinc Oxide Nanoparticles Inhibition of Initial Adhesion and ALS1 and ALS3 Gene Expression in Candida albicans Strains from Urinary Tract Infections.

Mycopathologia. 2019-3-22

[2]
The Etest Performed Directly on Blood Culture Bottles Is a Reliable Tool for Detection of Fluconazole-Resistant Candida albicans Isolates.

Antimicrob Agents Chemother. 2017-6-27

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Antimicrob Agents Chemother. 2013-3-25

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Antimicrob Agents Chemother. 2012-10-15

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PLoS One. 2012-3-30

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