通过多拷贝质粒上的四个大肠杆菌基因组区域对丝裂霉素C作用进行体内滴定。
In vivo titration of mitomycin C action by four Escherichia coli genomic regions on multicopy plasmids.
作者信息
Wei Y, Vollmer A C, LaRossa R A
机构信息
Biochemical Science and Engineering, Central Research and Development, DuPont Company, Wilmington, Delaware 19880-0173, USA.
出版信息
J Bacteriol. 2001 Apr;183(7):2259-64. doi: 10.1128/JB.183.7.2259-2264.2001.
Abstract
Mitomycin C (MMC), a DNA-damaging agent, is a potent inducer of the bacterial SOS response; surprisingly, it has not been used to select resistant mutants from wild-type Escherichia coli. MMC resistance is caused by the presence of any of four distinct E. coli genes (mdfA, gyrl, rob, and sdiA) on high-copy-number vectors. mdfA encodes a membrane efflux pump whose overexpression results in broad-spectrum chemical resistance. The gyrI (also called sbmC) gene product inhibits DNA gyrase activity in vitro, while the rob protein appears to function in transcriptional activation of efflux pumps. SdiA is a transcriptional activator of ftsQAZ genes involved in cell division.
摘要
丝裂霉素C（MMC）是一种DNA损伤剂，是细菌SOS反应的强效诱导剂；令人惊讶的是，它尚未被用于从野生型大肠杆菌中筛选抗性突变体。MMC抗性是由高拷贝数载体上四个不同的大肠杆菌基因（mdfA、gyrl、rob和sdiA）中的任何一个的存在引起的。mdfA编码一种膜外排泵，其过表达导致广谱化学抗性。gyrI（也称为sbmC）基因产物在体外抑制DNA回旋酶活性，而rob蛋白似乎在激活外排泵的转录中发挥作用。SdiA是参与细胞分裂的ftsQAZ基因的转录激活因子。
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2
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J Bacteriol. 2000 Jul;182(13):3794-801. doi: 10.1128/JB.182.13.3794-3801.2000.
3
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Mutat Res. 2000 Mar 3;466(1):97-107. doi: 10.1016/s1383-5718(99)00233-8.
4
Mitomycin C linked to DNA minor groove binding agents: synthesis, reductive activation, DNA binding and cross-linking properties and in vitro antitumor activity.与DNA小沟结合剂相连的丝裂霉素C：合成、还原激活、DNA结合和交联特性以及体外抗肿瘤活性。
Bioorg Med Chem. 1999 Dec;7(12):2713-26. doi: 10.1016/s0968-0896(99)00223-0.
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Pharmacol Ther. 1997 Oct-Dec;76(1-3):73-87. doi: 10.1016/s0163-7258(97)00088-0.
6
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J Bacteriol. 1997 Oct;179(19):6122-6. doi: 10.1128/jb.179.19.6122-6126.1997.
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