Yamaoka A, Matsuo T, Shiraga F, Ohtsuki H
Department of Ophthalmology, Okayama University Medical School, Okayama, Japan.
Ophthalmic Res. 2001 Mar-Apr;33(2):98-101. doi: 10.1159/000055651.
The effect of mechanical stretching was examined on cultured scleral fibroblasts of the human eye in order to observe changes in their production of TIMP (tissue inhibitor of metalloproteinase)-1, MMP (matrix metalloproteinase)-1 and -2 in response to physiological strain.
Human scleral fibroblasts were cultured from scleral tissue resected during foveal translocation surgery. The fibroblasts in near confluency were exposed to mechanical stretching of the bottom of a 6-cm Petri dish at the maximum magnitude of 4500 microstrain and at a cycle of 30 s for 72 h. TIMP-1, MMP-1 and MMP-2 levels in the medium following 24, 48 and 72 h of cyclic stretching were measured by enzyme immunoassay.
The growth of scleral fibroblasts during the 72-hour period of stretching did not show a significant difference from that of non-stretched control fibroblasts. Scleral fibroblasts in the stretched group produced a significantly smaller amount of TIMP-1 at 72 h after stretching, compared with nonstretched control (p = 0.0353, Student t-test). The levels of MMP-1 and MMP-2 produced by scleral fibroblasts were not significantly different between the stretched group and nonstretched group.
The production of TIMP-1 by human scleral fibroblasts was suppressed by cyclic mechanical stretching. Mechanical strain would be one factor to regulate the homeostasis of extracellular matrix in the sclera.
研究机械拉伸对人眼培养的巩膜成纤维细胞的影响，以观察其在生理应变下金属蛋白酶组织抑制剂（TIMP）-1、基质金属蛋白酶（MMP）-1和-2产生的变化。
从黄斑转位手术中切除的巩膜组织培养人巩膜成纤维细胞。将接近汇合的成纤维细胞暴露于6厘米培养皿底部的机械拉伸，最大应变幅度为4500微应变，周期为30秒，持续72小时。通过酶免疫测定法测量循环拉伸24、48和72小时后培养基中TIMP-1、MMP-1和MMP-2的水平。
在72小时的拉伸期间，巩膜成纤维细胞的生长与未拉伸的对照成纤维细胞相比没有显著差异。与未拉伸对照相比，拉伸组的巩膜成纤维细胞在拉伸72小时后产生的TIMP-1量显著减少（p = 0.0353，Student t检验）。拉伸组和未拉伸组的巩膜成纤维细胞产生的MMP-1和MMP-2水平没有显著差异。
循环机械拉伸抑制了人巩膜成纤维细胞TIMP-1的产生。机械应变可能是调节巩膜细胞外基质稳态的一个因素。
