Sehgal S C, Vijayachari P, Smythe L D, Norris M, Symonds M, Dohnt M, Korver H, v d Kemp H, Hartskeerl R A, Terpstra W J
Regional Medical Research Centre (ICMR), Port Blair, Australia.
Indian J Med Res. 2000 Oct;112:135-9.
BACKGROUND & OBJECTIVES: Leptospirosis has been an important public health problem in the Andaman Islands since 1988. As information about the exact etiological agent is not available, the present study was undertaken to isolate and identify Leptospira from human patients.
An isolate coded AF61 was recovered from the blood of a patient clinically suspected to have leptospirosis, with fever, headache and body aches as the main symptoms. The isolation was done using Ellinghausen-McCullough-Johnson-Harris (EMJH) medium following standard procedure. The isolate was identified using microscopic agglutination test (MAT) with 'groupsera', cross agglutination absorption test (CAAT) and monoclonal antibodies.
Agglutination tests with rabbit antisera revealed that the isolate belonged to the serogroup icterohaemorrhagiae. The CAAT results showed that it was closely related to the serovar lai. Analysis of AF61 with monoclonal antibodies confirms our observation with CAAT that it is closely related to the reference strain Lai serovar lai.
INTERPRETATION & CONCLUSIONS: Serovar lai, has been associated with pulmonary haemorrhage in China and Korea. However, the strain AF61 was not isolated from a patient with pulmonary symptoms. Further studies are needed to understand the possible relationship between serovars and clinical patterns and the distribution of serovar lai and lai-like strains in Asia.
自1988年以来，钩端螺旋体病一直是安达曼群岛的一个重要公共卫生问题。由于缺乏关于确切病原体的信息，本研究旨在从人类患者中分离和鉴定钩端螺旋体。
从一名临床疑似钩端螺旋体病患者的血液中分离出一株编号为AF61的菌株，该患者的主要症状为发热、头痛和全身疼痛。按照标准程序，使用埃林豪森-麦卡洛-约翰逊-哈里斯（EMJH）培养基进行分离。使用显微镜凝集试验（MAT）、交叉凝集吸收试验（CAAT）和单克隆抗体对分离株进行鉴定。
与兔抗血清的凝集试验表明，该分离株属于黄疸出血群。CAAT结果显示，它与赖型血清型密切相关。用单克隆抗体对AF61进行分析，证实了我们在CAAT中的观察结果，即它与参考菌株赖型赖血清型密切相关。
在中国和韩国，赖型血清型与肺出血有关。然而，AF61菌株并非从有肺部症状的患者中分离得到。需要进一步研究以了解血清型与临床模式之间的可能关系，以及亚洲赖型血清型和类赖菌株的分布情况。
