Naito Z, Takashi E, Asano G
Department of Pathology, Nippon Mecical School, Tokyo, Japan 113.
Zhonghua Bing Li Xue Za Zhi. 1998 Feb;27(1):35-9.
To study the role of heat shock proteins (HSP) in the cell cycle and various processes of carcinogenesis.
Immunohistochemical SP methods, electron microscopy, in situ hybridization and RT-PCR were used to evaluate the expression of HSP, mainly HSP90, ubiquitin and HSP70 in breast cancer tissues.
HSP90 mRNA was expressed at much higher levels in cancerous tissue than in non-cancerous tissues. In addition, a close relation between HSP90 mRNA expression and proliferating cell nuclear antigen labelling index (PCNA L. I.) was observed in cancerous tissue. These findings suggest that increased expression of HPS90 isoform may play a role in cell proliferation. On the other hand, HSP90 mRNA was expressed in the more poorly differentiated carcinomas of the breast. The intracellular localization of HSP70 was consistent with that of ubiquitin. The PCNA L. I. was significantly higher in specimens showing HSP70 in nucleus. HSP73 mRNA, a member of HSP70 family, was also expressed at higher levels in cancerous tissues associated with a high PCNA L. I. than in non-cancerous tissues.
These results suggest that HSP90 may play a role in cancer cell proliferation and that HSP90 may contribute to cell differentiation and structural constitution. In addition, HSP70, especially HSP73, is related to ubiquetin and seems to be a marker for cancer proliferation.
研究热休克蛋白（HSP）在细胞周期及肿瘤发生的各个过程中的作用。
采用免疫组织化学SP法、电子显微镜、原位杂交及逆转录-聚合酶链反应（RT-PCR）来评估乳腺癌组织中HSP，主要是HSP90、泛素和HSP70的表达。
HSP90 mRNA在癌组织中的表达水平远高于非癌组织。此外，在癌组织中观察到HSP90 mRNA表达与增殖细胞核抗原标记指数（PCNA L.I.）之间存在密切关系。这些发现表明HPS90异构体表达的增加可能在细胞增殖中起作用。另一方面，HSP90 mRNA在分化较差的乳腺癌中表达。HSP70的细胞内定位与泛素一致。在细胞核中显示HSP70的标本中，PCNA L.I.显著更高。HSP70家族成员HSP73 mRNA在癌组织中的表达水平也高于非癌组织，且与高PCNA L.I.相关。
这些结果表明HSP90可能在癌细胞增殖中起作用，并且HSP90可能有助于细胞分化和结构构成。此外，HSP70，尤其是HSP73，与泛素相关，似乎是癌症增殖的一个标志物。
