[Effects of oxidatively modified lipoproteins on the expression of monocyte chemoattractant protein-1 in endothelial cells].

OBJECTIVE

To clarify whether lipoproteins, particularly oxidatively modified low density lipoprotein (OX-LDL) and very low density lipoprotein (OX-VLDL) play a role in the express of monocyte chemoattractant protein-1 (MCP-1) mRNA and protein in endothelial cells (ECs).

METHODS

After a 24-hour exposure to LDL, OX-LDL, VLDL and OX-VLDL respectively the total RNA in call aorta ECs was extracted by means of guanidinium isothiocyanate method. MCP-1 mRNA expression in ECs was examined by dot blot analysis using a gamma-32P-end-labelled 35 mer oligonucleotide probe of MCP-1. Meanwhile, MCP-1 protein in EC conditioned media (EC-CM) of each group was determined by sandwich ELISA, and MCP-1 protein in ECs was examined immunocytochemically as well.

RESULTS

Cultured calf aorta ECs expressed MCP-1 mRNA and protein, and OX-LDL and OX-VLDL induced further a strong expression of MCP-1 mRNA, and an increased MCP-1 protein level in EC-CM. The expression of MCP-1 mRNA and protein was only slightly increased when exposed to LDL and VLDL, and immunohistological staining with polyclonal MCP-1 antibody give a similar result.

CONCLUSION

OX-LDL and OX-VLDL are able to induce a strong expression of MCP-1 in ECs.