Wang G, Deng Z, Ni J
Department of Pathology, Tongji Medical University, Wuhan.
J Tongji Med Univ. 1997;17(4):203-5. doi: 10.1007/BF02895619.
Monocyte chemoattractant protein-1 (MCP-1), a potent chemoattractant, is thought to play an important role in migration of monocytes into atherosclerotic lesions. The present study was designed to investigate the capacity of human peripheral blood monocytes to express MCP-1 and effects of native very low density lipoprotein (VLDL) and oxidized VLDL (OX-VLDL) on the expression. The total RNA was extracted from cultured monocytes, which were exposed to VLDL and OX-VLDL, and the media conditioned by monocytes were collected. MCP-1 mRNA expression was examined by Northern blot analysis. MCP-1 protein in conditioned media was determined by using sandwich ELISA. The results showed that monocytes can express MCP-1 after a 24 h incubation at 37 degrees C, and the expression was markedly increased by a exposure to OX-VLDL, whereas the expression was slightly increased when exposed to VLDL. It suggests that the capacity of monocytes to produce MCP-1 that recruits and activates circulating monocytes may be of considerable importance in atherogenesis, and oxidation of VLDL enhances its potential to promote atherogenesis.
单核细胞趋化蛋白-1（MCP-1）是一种强效趋化因子，被认为在单核细胞迁移至动脉粥样硬化病变过程中起重要作用。本研究旨在调查人外周血单核细胞表达MCP-1的能力，以及天然极低密度脂蛋白（VLDL）和氧化极低密度脂蛋白（OX-VLDL）对该表达的影响。从培养的单核细胞中提取总RNA，这些单核细胞分别暴露于VLDL和OX-VLDL，收集单核细胞条件培养基。通过Northern印迹分析检测MCP-1 mRNA表达。使用夹心ELISA法测定条件培养基中的MCP-1蛋白。结果显示，单核细胞在37℃孵育24小时后可表达MCP-1，暴露于OX-VLDL时该表达显著增加，而暴露于VLDL时表达略有增加。这表明单核细胞产生MCP-1以募集和激活循环单核细胞的能力在动脉粥样硬化形成中可能相当重要，且VLDL的氧化增强了其促进动脉粥样硬化的潜力。
