Urasaki N, Tokumoto M, Terauchi R, Tarora K, Chinen I, Ban Y, Kayano T, Tanaka H
Iwate Biotechnology Research Center, Kitakami, Japan.
Genes Genet Syst. 2000 Oct;75(5):293-8. doi: 10.1266/ggs.75.293.
In the course of papaya EST collection, one clone (pRA4-3) encoding partial sequence of papaya small GTP-binding protein gene, pgp1, was obtained. Based on the sequence information of pRA4-3, the entire coding region of pgp1 was cloned using the 3'RACE PCR technique. ORF of pgp1 is 636bp long and deduced molecular weight of the protein is 23,311. Phylogenetic analysis showed that PGP1 belongs to YPT/RAB group of the small GTP-binding protein and is a homologue of RAB2. Southern analysis showed that there are several pgp1-related genes in papaya genome. Northern analysis showed that pgp1 was expressed equally in stems of seedlings that were grown under light and dark conditions. This result shows that PGP1 is not involved in the phytochrome-mediated signal transduction as an auxin signal transducer in stems of papaya seedlings.
在番木瓜表达序列标签（EST）收集过程中，获得了一个编码番木瓜小GTP结合蛋白基因pgp1部分序列的克隆（pRA4-3）。基于pRA4-3的序列信息，使用3'RACE PCR技术克隆了pgp1的完整编码区。pgp1的开放阅读框（ORF）长636bp，推导的蛋白质分子量为23311。系统发育分析表明，PGP1属于小GTP结合蛋白的YPT/RAB组，是RAB2的同源物。Southern分析表明，番木瓜基因组中有几个与pgp1相关的基因。Northern分析表明，pgp1在光照和黑暗条件下生长的幼苗茎中表达水平相同。该结果表明，在番木瓜幼苗茎中，PGP1不作为生长素信号转导器参与光敏色素介导的信号转导。
