编码一种新型小GTP结合蛋白的cDNA的分子克隆

Molecular cloning of a cDNA encoding a novel small GTP-binding protein.

作者信息

Koda T, Kakinuma M

机构信息

Section of Bacterial Infection, Hokkaido University, Sapporo, Japan.

出版信息

FEBS Lett. 1993 Aug 9;328(1-2):21-4. doi: 10.1016/0014-5793(93)80957-v.

DOI:10.1016/0014-5793(93)80957-v

PMID:7688322

Abstract

A cDNA encoding a small GTP-binding protein, S10, was cloned from Jurkat cells. The deduced amino acid sequence of S10 had the structural features characteristic to this family of proteins with highest homology to rab subfamily. Northern blot analysis revealed that this gene is expressed only in lymphoid cell lines and a histiocytic leukemia, U937. Hence, it should have a specialized function in cells derived from the hematopoietic stem cell.

摘要

从Jurkat细胞中克隆出一个编码小GTP结合蛋白S10的cDNA。S10推导的氨基酸序列具有该蛋白家族的结构特征，与rab亚家族的同源性最高。Northern印迹分析表明，该基因仅在淋巴样细胞系和组织细胞白血病U937中表达。因此，它在源自造血干细胞的细胞中应具有特定功能。