Yan H, Bunge M B, Wood P M, Plant G W
The Miami Project to Cure Paralysis, University of Miami School of Medicine, Miami, FL 33101, USA.
Glia. 2001 Mar 15;33(4):334-42. doi: 10.1002/1098-1136(20010315)33:4<334::aid-glia1032>3.0.co;2-i.
Olfactory ensheathing glia (EG) from adult rat proliferate slowly in vitro without added mitogens. The potential future use of EG in transplantation within the central nervous system to improve neural repair is dependent on identifying mitogens that will effectively expand EG without altering their phenotype. The mitogenic effects of heregulin (HRG), fibroblast growth factor 2 (FGF-2), platelet-derived growth factor BB (PDGF-BB), insulin-like growth factor 1 (IGF-1), and forskolin (FSK) on cultured adult-derived rat EG were monitored by tritiated-thymidine labeling and p75 immunostaining. In serum-containing medium, HRG, FGF-2, PDGF-BB, IGF-1, and FSK were capable of stimulating EG proliferation, and the stimulation by these growth factors was potentiated by FSK. The combinations of HRG + FGF-2, HRG + PDGF-BB, HRG + IGF-1, FGF-2 + PDGF-BB, and FGF-2 + IGF-1 all promoted EG proliferation in an additive manner. In serum-free medium, HRG and FGF-2 were mitogenic, but PDGF-BB, IGF-1 and FSK were not; however, FSK potentiated the stimulation by HRG and FGF-2, and the combination of HRG + FGF-2 promoted EG proliferation in an additive manner. This new information will be useful for the design of protocols to achieve sufficient numbers of adult-derived EG for clinical purposes. This study also further establishes similarities between EG and Schwann cells.
成年大鼠的嗅鞘胶质细胞（EG）在无添加促有丝分裂原的体外环境中增殖缓慢。EG未来在中枢神经系统移植中用于改善神经修复的可能性，取决于能否识别出能有效扩增EG且不改变其表型的促有丝分裂原。通过氚标记胸腺嘧啶核苷标记和p75免疫染色监测了这里调节蛋白（HRG）、成纤维细胞生长因子2（FGF - 2）、血小板衍生生长因子BB（PDGF - BB）、胰岛素样生长因子1（IGF - 1）和福斯高林（FSK）对培养的成年大鼠来源EG的促有丝分裂作用。在含血清培养基中，HRG、FGF - 2、PDGF - BB、IGF - 1和FSK能够刺激EG增殖，并且这些生长因子的刺激作用被FSK增强。HRG + FGF - 2、HRG + PDGF - BB、HRG + IGF - 1、FGF - 2 + PDGF - BB和FGF - 2 + IGF - 1的组合均以相加的方式促进EG增殖。在无血清培养基中，HRG和FGF - 2具有促有丝分裂作用，但PDGF - BB、IGF - 1和FSK则没有；然而，FSK增强了HRG和FGF - 2的刺激作用，并且HRG + FGF - 2的组合以相加的方式促进EG增殖。这一新信息将有助于设计方案，以获得足够数量用于临床目的的成年大鼠来源EG。本研究还进一步证实了EG与雪旺细胞之间的相似性。
